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Catalog Number Size Price
LS-C353340-100 100 µl (1 mg/ml) $299 
PARK7 / DJ-1 Antibody - Immunohistochemical analysis of DJ-1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
PARK7 / DJ-1 Antibody - Immunofluorescent analysis of DJ-1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
PARK7 / DJ-1 Antibody - Western blot analysis of DJ-1 expression in HeLa (A); HEK293T (B); Jurkat (C) whole cell lysates.
PARK7 / DJ-1 Antibody - Immunohistochemical analysis of DJ-1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
PARK7 / DJ-1 Antibody - Immunofluorescent analysis of DJ-1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
PARK7 / DJ-1 Antibody - Western blot analysis of DJ-1 expression in HeLa (A); HEK293T (B); Jurkat (C) whole cell lysates.
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Polyclonal Rabbit anti‑Human PARK7 / DJ‑1 Antibody (Internal, IHC, IF, WB) LS‑C353340

Polyclonal Rabbit anti‑Human PARK7 / DJ‑1 Antibody (Internal, IHC, IF, WB) LS‑C353340

Antibody:
PARK7 / DJ-1 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Bovine, Pig
Format:
Unconjugated, Unmodified
Price
Catalog Number
$299
LS-C353340-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
PARK7 / DJ-1 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Bovine, Pig
Format:
Unconjugated, Unmodified

Specifications

Description
DJ-1 antibody LS-C353340 is an unconjugated rabbit polyclonal antibody to DJ-1 (PARK7) (Internal) from human. It is reactive with human, bovine and pig. Validated for ICC, IF, IHC and WB. Cited in 1 publication.
Target
Human PARK7 / DJ-1
Synonyms
PARK7 | DJ-1 | DJ1 | Parkinson disease protein 7 | Park-7 | Oncogene DJ1 | Parkinson protein 7 | Protein DJ-1
Host
Rabbit
Reactivity
Human, Bovine, Pig (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the central region of human DJ-1.
Epitope
Internal
Specificity
Recognizes endogenous levels of DJ-1 protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Store at -20°C. Aliquot to avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PARK7 / DJ-1
Q99497 NM_007262 NP_009193.2

LSBio Ratings

PARK7 / DJ-1 Antibody (Internal) for IHC, ICC, IF/Immunofluorescence, WB/Western LS-C353340 has an LSBio Rating of
Publications (4)

Publications (1)

Parkinson Disease Protein 7 (PARK7) Is Related to the Ability of Mammalian Sperm to Undergo In Vitro Capacitation. Sandra Recuero , Ariadna Delgado-Bermúdez , Yentel Mateo-Otero , Estela Garcia-Bonavila , Marc Llavanera , Marc Yeste. International journal of molecular sciences. 2021 Oct;22:10804.

Customer Reviews (0)


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Requested From: United States
Date Requested: 11/7/2024