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Anti-OCLN / Occludin Antibody (C-Terminus, clone OC-3F10) IHC-plus™ LS-B2320

Note: This antibody replaces LS-C37845

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml Unavailable

Most Popular OCLN / Occludin Antibodies

Anti-OCLN / Occludin Antibody (C-Terminus) IHC-plus™ LS-B2187
Rabbit Polyclonal (IgG) to Human OCLN / Occludin
Human, Mouse, Rat, Dog
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image
Anti-OCLN / Occludin Antibody (C-Terminus, clone OC-3F10) IHC-plus™ LS-B2320
Mouse Monoclonal [clone OC-3F10] (IgG1,k) to Human OCLN / Occludin
Human, Mouse, Rat, Dog
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-OCLN / Occludin Antibody (C-Terminus) LS-C108451
Rabbit Polyclonal to Human OCLN / Occludin
Human, Mouse, Rat
IHC, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone OC-3F10] (IgG1,k) to Human OCLN / Occludin
Human, Mouse, Rat, Dog
IHC - Paraffin, Immunofluorescence, Western blot, ELISA (applications tested for the base form of this product only)
Unconjugated

Details

Human OCLN / Occludin
Mouse
Human, Mouse, Rat, Dog (tested or 100% immunogen sequence identity)
IgG1,k Monoclonal [OC-3F10]
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, MaxLight 405, MaxLight 490, MaxLight 550, MaxLight 650, MaxLight 750, PE.
Protein A purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • Immunofluorescence (2 - 3 µg/ml)
  • Western blot (0.1 - 1 µg/ml)
  • ELISA (0.1 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

OCLN / Occludin antibody was raised against gST fusion protein consisting of the C-terminal region (~150aa) of human occludin.
C-Terminus
Recognizes mammalian Occludin Protein. Species cross-reactivity: human, mouse, rat and canine.
Immunohistochemistry: LS-B2320 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2320 was determined to be 10 ug/ml. Positive control: T84 cell line (human intestinal epithelium), MDCK cells (canine kidney), rat liver and Caco-2 cells (human colon adenocarcinoma). Does not appear to detect the most highly phosphorylated form of the occludin protein when tested on Caco-2 cells.

Packaging

PBS, pH 7.4, 0.09% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About OCLN / Occludin

Q16625 NM_002538 NP_002529.1

OCLN Antibody, Occludin Antibody, BLCPMG Antibody

OCLN / Occludin is an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants.

Immunohistochemistry

Immunohistochemistry
Anti-Occludin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2320 concentration 10 ug/ml.
 

Requested From: 
Date Requested: 3/28/2017

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