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Catalog Number Size Price
LS-C37846-100 100 µg $919 
Antibody

Monoclonal Mouse anti‑Human OCLN / Occludin Antibody (clone OC‑3F10, FITC, C‑Terminus, IF, WB) LS‑C37846

Monoclonal Mouse anti‑Human OCLN / Occludin Antibody (clone OC‑3F10, FITC, C‑Terminus, IF, WB) LS‑C37846

Antibody:
OCLN / Occludin Mouse anti-Human Monoclonal (C-Terminus) (FITC) (OC-3F10) Antibody
Application:
IF, WB, ELISA
Reactivity:
Human, Mouse, Rat, Dog
Format:
FITC, Unmodified
Price
Catalog Number
$919
LS-C37846-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
OCLN / Occludin Mouse anti-Human Monoclonal (C-Terminus) (FITC) (OC-3F10) Antibody
Application:
IF, WB, ELISA
Reactivity:
Human, Mouse, Rat, Dog
Format:
FITC, Unmodified

Specifications

Description
Occludin antibody LS-C37846 is an FITC-conjugated mouse monoclonal antibody to Occludin (OCLN) (C-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IF and WB.
Target
Human OCLN / Occludin
Synonyms
OCLN | Occludin | BLCPMG
Host
Mouse
Reactivity
Human, Mouse, Rat, Dog (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
OC-3F10
Conjugations
FITC
Purification
Protein A purified
Modifications
Unmodified
Immunogen
GST fusion protein consisting of the C-Terminus (~150aa) of human occludin.
Epitope
C-Terminus
Specificity
Recognizes human Occludin Protein. Species cross-reactivity: mouse, rat and canine.
Applications
  • Immunofluorescence (2 - 10 µg/ml)
  • Western blot (0.1 - 1 µg/ml)
  • ELISA (0.1 - 1 µg/ml)
Usage
Suitable for use in ELISA, Western Blot and Immunofluorescence. ELISA: 0.1-1 ug/ml. Western Blot: Detects a band at ~65kD. Immunofluorescence: 2-10 ug/ml. Positive control: T84 cell line (human intestinal epithelium), MDCK cells (canine kidney), Caco-2 cells (human colon adenocarcinoma) and rat liver. Does not appear to detect the most highly phosphorylated form of the occludin protein when tested on Caco-2 cells. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol, 1% BSA
Storage
Short term: 4°C; Long term: -20°C; Protect from light.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About OCLN / Occludin
Q16625 NM_002538 NP_002529.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/18/2024