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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
Protein Family And Group
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Catalog Number Size Price
LS-B2187-50 50 µg (0.25 mg/ml) $485 
OCLN / Occludin Antibody - Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)
OCLN / Occludin Antibody - Anti-Occludin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
OCLN / Occludin Antibody - Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)
OCLN / Occludin Antibody - Anti-Occludin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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PathPlus™ Polyclonal Rabbit anti‑Human OCLN / Occludin Antibody (C‑Terminus, IHC, IF, WB) LS‑B2187

PathPlus™ Polyclonal Rabbit anti‑Human OCLN / Occludin Antibody (C‑Terminus, IHC, IF, WB) LS‑B2187

Note: This antibody replaces LS-C37847
Antibody:
Rabbit Polyclonal to Human OCLN / Occludin
Application:
IHC-P, IF, WB, IP, ELISA
Reactivity:
Human, Mouse, Rat, Dog
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$485 
LS-B2187-50
50 µg
0.25 mg/ml
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Rabbit Polyclonal to Human OCLN / Occludin
Application:
IHC-P, IF, WB, IP, ELISA
Reactivity:
Human, Mouse, Rat, Dog
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
OCLN (Occludin) is an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5. References: The UniProt Consortium. Nucleic Acids Res. 47: D506-515 (2019); Nucleic Acids Res. 2016 Jan 4;44(D1):D733-45, PMID:26553804
Target
Human OCLN / Occludin
Synonyms
OCLN | Occludin | BLCPMG
Host
Rabbit
Reactivity
Human, Mouse, Rat, Dog (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC - Paraffin (2.5 µg/ml)
  • Immunofluorescence
  • Western blot (1 - 3 µg/ml)
  • Immunoprecipitation
  • ELISA (0.1 - 1 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
A GST fusion protein consisting of the C-Terminus 150 amino acids of human occludin fused to GST.
Epitope
C-Terminus
Specificity
Recognizes human occludin at ~65kD. Reactivity of this antibody with the occludin protein has been confirmed by Western Blot using total cell lysates derived from MDCK cells, human T84 cells, human Caco-2 and rat fibroblasts transfected with a plasmid encoding human occludin. Species cross-reactivity has been confirmed in Western blot only.
Usage
Immunohistochemistry: LS-B2187 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2187 was determined to be 2.5 ug/ml.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About OCLN / Occludin
Q16625 NM_002538 NP_002529.1

Validation

OCLN / Occludin Antibody - Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)

OCLN / Occludin Antibody - Anti-Occludin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-Occludin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-Occludin antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

LSBio Ratings

PathPlus™ OCLN / Occludin Antibody (C-Terminus) for IHC, IF/Immunofluorescence, WB/Western, IP, ELISA LS-B2187 has an LSBio Rating of
Publications (4.2)
Laboratory Validation Score (5)

Publications (3)

The tight-junction protein claudin-6 induces epithelial differentiation from mouse F9 and embryonic stem cells. Sugimoto K, Ichikawa-Tomikawa N, Satohisa S, Akashi Y, Kanai R, Saito T, Sawada N, Chiba H. PloS one. 2013 8:e75106.

ATF3 Protects Pulmonary Resident Cells from Acute and Ventilator-Induced Lung Injury by Preventing Nrf2 Degradation. Shan Y, Akram A, Amatullah H, Zhou DY, Gali PL, Maron-Gutierrez T, Gonz[Character e1]lez-L[Character f3]pez A, Zhou L, Rocco PR, Hwang D, Albaiceta GM, Haitsma JJ, Dos Santos CC. Antioxidants & redox signaling. 2014 Mar;22:651-68.

TLR3 deficiency exacerbates the loss of epithelial barrier function during genital tract Chlamydia muridarum infection. Kumar R, Gong H, Liu L, Ramos-Solis N, Seye CI, Derbigny WA. PloS one. 2019 January;14:e0207422.

Customer Reviews (0)


Requested From: United States
Date Requested: 7/5/2020