Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Western blot, Immunoprecipitation, ELISA, Gel shift
Human, Mouse (tested or 100% immunogen sequence identity)
Western blot (1:1000)
Specificity and Use
NFYB antibody was raised against nF-Y (A subunit) peptide corresponding to a region near the N-terminus conjugated to KLH.
Recognizes human Nuclear transcription factor Y subunit alpha, also known as NF-Y subunit a, NF-YA or CAAT box DNA-binding protein subunit A. Recognizes both differentially spliced 35 kD and 42 kD forms of the A subunit of NF-Y but demonstrates no reactivity against the 25 kD B subunit.
NFYB is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif.