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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NFYB Antibody LS‑C346341
NFYB antibody LS-C346341 is an unconjugated rabbit polyclonal antibody to NFYB from human and mouse. Validated for IF, IHC and WB.
Catalog
Size
Price
LS-C346341-50
50 µl
$235
LS-C346341-100
100 µl
$315
LS-C346341-200
200 µl
$425

Popular NFYB Products

Anti-NFYB antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B718 dilution 1:500.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Transfected 293T Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken
Applications: Western blot
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-NFYB mAb.
Species: Human
Applications: ICC, Immunofluorescence, Western blot, Dot Blot
Antibody
Species: Human, Mouse
Applications: Western blot, Immunoprecipitation, ELISA, Gel shift

Product Description

NFYB Antibody for IHC, IF/Immunofluorescence, WB/Western LS-C346341

Specifications

Target
Human NFYB
Synonyms
NFYB, CBF-A, CBF-B, HAP3, NF-YB
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:50 - 1:100)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
NFYB antibody was raised against recombinant fusion protein containing a sequence corresponding to amino acids 1-207 of human NFYB (NP_006157.1). MTMDGDSSTTDASQLGISADYIGGSHYVIQPHDDTEDSMNDHEDTNGSKESFREQDIYLPIANVARIMKNAIPQTGKIAKDAKECVQECVSEFISFITSEASERCHQEKRKTINGEDILFAMSTLGFDSYVEPLKLYLQKFREAMKGEKGIGGAVTATDGLSEELTEEAFTNQLPAGLITTDGQQQNVMVYTTSYQQISGVQQIQFS
Specificity
Human NFYB
Usage
The predicted MW is 22kDa, while the observed MW by Western blot was 28kDa.
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About NFYB
NFYB is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. P25208 NM_006166 NP_006157.1


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Images

Immunofluorescence

Immunofluorescence analysis of MCF7 cells.
Immunofluorescence analysis of MCF7 cells.

Western blot

Western blot analysis of extracts of various cell lines.
Western blot analysis of extracts of various cell lines.

Immunofluorescence

Immunofluorescence analysis of MCF7 cells.
Immunofluorescence analysis of MCF7 cells.

Western blot

Western blot analysis of extracts of various cell lines.
Western blot analysis of extracts of various cell lines.

Immunofluorescence

Immunofluorescence analysis of MCF7 cells.
Immunofluorescence analysis of MCF7 cells.

Western blot

Western blot analysis of extracts of various cell lines.
Western blot analysis of extracts of various cell lines.

Immunofluorescence

Immunofluorescence analysis of MCF7 cells.
Immunofluorescence analysis of MCF7 cells.

Western blot

Western blot analysis of extracts of various cell lines.
Western blot analysis of extracts of various cell lines.

Requested From: United States
Date Requested: 10/20/2018

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