LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us
NFYB Antibody (C‑Terminus) LS‑C109581
NFYB antibody LS-C109581 is an unconjugated rabbit polyclonal antibody to NFYB from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C109581-50
50 µg
$365

Popular NFYB Products

Anti-NFYB antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B718 dilution 1:500.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Transfected 293T Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken
Applications: Western blot
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-NFYB mAb.
Species: Human
Applications: ICC, Immunofluorescence, Western blot, Dot Blot
Immunofluorescence analysis of MCF7 cells.
Species: Human, Mouse
Applications: IHC, Immunofluorescence, Western blot
Antibody
Species: Human, Mouse
Applications: Western blot, Immunoprecipitation, ELISA, Gel shift

Product Description

NFYB antibody LS-C109581 is an unconjugated rabbit polyclonal antibody to NFYB from human, mouse, rat and other species. Validated for WB.

Specifications

Target
Human NFYB
Synonyms
NFYB, CBF-A, CBF-B, HAP3, NF-YB
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Dog, Guinea pig, Pig, Rabbit, Chicken (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Immunogen
NFYB antibody was raised against synthetic peptide from C-Terminus of human NFYB (P25208, NP_006157). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Rabbit, Pig, Opossum, Turkey, Chicken (100%); Lizard (93%); Bovine, Horse, Platypus (92%); Guinea pig (85%).
Blocking Peptide
LS-E10912 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C109581. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Human NFYB
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS, 0.09% sodium azide, 2% sucrose
Reconstitution
Centrifuge the vial prior to opening. Reconstitute with sterile distilled water to a concentration of 1 mg/ml. Vortex and centrifuge again.
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About NFYB
NFYB is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. P25208 NM_006166 NP_006157.1


Publications (0)


Customer Reviews (0)


Images

Western blot

NFYB antibody LS-C109581 Western blot of Transfected 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
NFYB antibody LS-C109581 Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

NFYB antibody LS-C109581 Western blot of Transfected 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
NFYB antibody LS-C109581 Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

NFYB antibody LS-C109581 Western blot of Transfected 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
NFYB antibody LS-C109581 Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

NFYB antibody LS-C109581 Western blot of Transfected 293T cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
NFYB antibody LS-C109581 Western blot of Transfected 293T cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: United States
Date Requested: 10/21/2018

Get Social With Us!
Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy