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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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NFYB Antibody (clone 6H6) LS‑C197778
NFYB antibody LS-C197778 is an unconjugated mouse monoclonal antibody to human NFYB. Validated for ELISA, RNAi and WB.
Catalog
Size
Price
LS-C197778-100
100 µg
Unavailable

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Product Description

NFYB antibody LS-C197778 is an unconjugated mouse monoclonal antibody to human NFYB. Validated for ELISA, RNAi and WB.
About NFYB
NFYB is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. P25208 NM_006166 NP_006157.1

NFYB Antibody, CBF-A Antibody, CBF-B Antibody, HAP3 Antibody, NF-YB Antibody


Specifications

Target
Human NFYB
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal [6H6]
Conjugations
Unconjugated
Purification
Purified from ascites by Protein A
Modifications
Unmodified
Applications
  • Western blot
  • ELISA
  • RNA interference
Immunogen
NFYB antibody was raised against nFYB (AAH05317, aa1-207 full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human NFYB
Usage
RNAi Knockdown: Antibody validated. Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate, Recombinant protein.
Presentation
PBS, pH 7.4
Storage
Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

Western Blot analysis of NFYB expression in transfected 293T cell line by NFYB monoclonal antibody (M01), clone 6H6.Lane 1: NFYB transfected lysate(22.8 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of NFYB expression in transfected 293T cell line by NFYB monoclonal antibody (M01), clone 6H6.Lane 1: NFYB transfected lysate(22.8 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged NFYB is approximately 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged NFYB is approximately 0.1 ng/ml as a capture antibody.

RNA interference

Western blot analysis of NFYB over-expressed 293 cell line, cotransfected with NFYB Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with NFYB monoclonal antibody (M01), clone 6H6 . GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western blot analysis of NFYB over-expressed 293 cell line, cotransfected with NFYB Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with NFYB monoclonal antibody (M01), clone 6H6 . GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western blot

Western Blot analysis of NFYB expression in transfected 293T cell line by NFYB monoclonal antibody (M01), clone 6H6.Lane 1: NFYB transfected lysate(22.8 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of NFYB expression in transfected 293T cell line by NFYB monoclonal antibody (M01), clone 6H6.Lane 1: NFYB transfected lysate(22.8 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged NFYB is approximately 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged NFYB is approximately 0.1 ng/ml as a capture antibody.

RNA interference

Western blot analysis of NFYB over-expressed 293 cell line, cotransfected with NFYB Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with NFYB monoclonal antibody (M01), clone 6H6 . GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western blot analysis of NFYB over-expressed 293 cell line, cotransfected with NFYB Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with NFYB monoclonal antibody (M01), clone 6H6 . GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western blot

Western Blot analysis of NFYB expression in transfected 293T cell line by NFYB monoclonal antibody (M01), clone 6H6.Lane 1: NFYB transfected lysate(22.8 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of NFYB expression in transfected 293T cell line by NFYB monoclonal antibody (M01), clone 6H6.Lane 1: NFYB transfected lysate(22.8 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged NFYB is approximately 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged NFYB is approximately 0.1 ng/ml as a capture antibody.

RNA interference

Western blot analysis of NFYB over-expressed 293 cell line, cotransfected with NFYB Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with NFYB monoclonal antibody (M01), clone 6H6 . GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western blot analysis of NFYB over-expressed 293 cell line, cotransfected with NFYB Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with NFYB monoclonal antibody (M01), clone 6H6 . GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western blot

Western Blot analysis of NFYB expression in transfected 293T cell line by NFYB monoclonal antibody (M01), clone 6H6.Lane 1: NFYB transfected lysate(22.8 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of NFYB expression in transfected 293T cell line by NFYB monoclonal antibody (M01), clone 6H6.Lane 1: NFYB transfected lysate(22.8 KDa).Lane 2: Non-transfected lysate.

Enzyme-Linked Immunosorbent Assay

Detection limit for recombinant GST tagged NFYB is approximately 0.1 ng/ml as a capture antibody.
Detection limit for recombinant GST tagged NFYB is approximately 0.1 ng/ml as a capture antibody.

RNA interference

Western blot analysis of NFYB over-expressed 293 cell line, cotransfected with NFYB Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with NFYB monoclonal antibody (M01), clone 6H6 . GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western blot analysis of NFYB over-expressed 293 cell line, cotransfected with NFYB Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with NFYB monoclonal antibody (M01), clone 6H6 . GAPDH ( 36.1 kDa ) used as specificity and loading control.


Requested From: 
Date Requested: 6/23/2018

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