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Anti-MYB / c-Myb Antibody (phospho-Thr486) LS-C153853

Ordering

Wt. Vol. Conc. Price
100 µg - - $405
Inquire for larger quantities

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Rabbit Polyclonal (IgG) to Mouse MYB / c-Myb
Mouse
Western blot, ELISA
Unconjugated

Details

Mouse MYB / c-Myb
Rabbit
Mouse (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Protein A purified
Unmodified

Applications

  • Western blot (1:2500)
  • ELISA

Specificity and Use

MYB / c-Myb antibody was raised against this affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of mouse c-Myb protein surrounding amino acid residue 486.
pThr486
This affinity-purified antibody is directed against the phosphorylated form of mouse c-Myb protein at the pT486 residue. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide. Reactivity occurs against mouse c-Myb pT486 protein and the antibody is specific for the phosphorylated form of the protein. This antibody reacts with endogenous c-Myb protein and also SUMOylated overexpressed c-Myb protein in transfected Cos7 cells. Reactivity with non-phosphorylated mouse c-Myb is minimal by ELISA and western blot. A BLAST analysis was used to suggest reactivity with c-Myb from mouse based on a 100% homology with the immunizing sequence. Expect reactivity with c-Myb from rat, pig, human, horse, platypus, opossum, orangutan, spider monkey, bonobo, gorilla, and chimpanzee; with a v-myb myeloblastosis viral oncogene homolog, all isoforms (also avian version) from human; and with a protooncogene c-myb, and a v-myb myeloblastosis viral oncogene homolog (avian), both from cattle, all based on a 93% homology with the immunizing sequence. Cross-reactivity with c-Myb from other sources has not been determined.
This affinity purified antibody has been tested for use in ELISA and western blotting using M1 cells which express endogenous wild-type c-Myb protein and in COS7 cells transfected with c-Myb. Specific conditions for reactivity and detection of phosphorylated c-Myb should be optimized by the end user. Expect a band approximately ~75 kD in size corresponding to phosphorylated c-Myb by western blotting in the appropriate cell lysate or extract.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide, sterile filtered
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About MYB / c-Myb

P10242 NM_005375 NP_005366.2

MYB Antibody, C-myb_CDS Antibody, C-myb8B_CDS Antibody, C-myb protein (140 AA) Antibody, C-myb10A_CDS Antibody, C-myb13A_CDS Antibody, Efg Antibody, Transcriptional activator Myb Antibody, C-myb Antibody, C-myb14A_CDS Antibody, Cmyb Antibody, Proto-oncogene c-Myb Antibody

MYB / c-Myb is a transcription factor that is a member of the MYB family of transcription factor genes. The protein contains three domains, an N-terminal DNA-binding domain, a central transcriptional activation domain and a C-terminal domain involved in transcriptional repression. This protein plays an essential role in the regulation of hematopoiesis and may play a role in tumorigenesis. Alternative splicing results in multiple transcript variants.

Western blot

Western blot
Western blot of Rabbit c-Myb pT486 antibody. Lane 1: Cos7 cells were either null for c-Myb. Lane 2: Cos7 cell lysates transfected with c-myb, no treatment. Lane 3: Cos7 cell lysates transfected with c-Myb and treated with okadaic acid to include phosphorylation of c-Myb. Load: 35 ug per lane. Primary antibody: The left blot was probed with anti-c-Myb antibody, the right blot was probed with anti-c-Myb pT486 antibody at 1:2500 for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10000 for 45 min at RT. Block: PBS-Tween (0.05%) overnight at 4C. Predicted/Observed size: 75, 98, and 125kDa corresponding to overexpressed c-Myb (arrow): wild-type, with one attached SUMO molecule, and with two attached SUMO molecules, respectively.
 

Requested From: United States
Date Requested: 12/5/2016

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