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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MID1IP1 Antibody (aa1‑183) LS‑C185843
MID1IP1 antibody LS-C185843 is an unconjugated rabbit polyclonal antibody to human MID1IP1. Validated for ICC, IHC and WB.
Catalog
Size
Price
LS-C185843-100
100 µl (0.68 mg/ml)
Unavailable

Popular MID1IP1 Products

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human MID1IP1 with C-terminal MYC tag probed with (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Prim
Species: Human, Monkey, Mouse, Rat, Bovine, Hamster, Horse, Pig, Rabbit, Chicken
Applications: Western blot, Peptide Enzyme-Linked Immunosorbent Assay
MID1IP1 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human stomach tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
Antibody
Species: Human
Applications: Western blot, Immunoprecipitation, ELISA
Antibody
Species: Human
Applications: Western blot, ELISA

Product Description

MID1IP1 antibody LS-C185843 is an unconjugated rabbit polyclonal antibody to human MID1IP1. Validated for ICC, IHC and WB.
About MID1IP1
Plays a role in the regulation of lipogenesis in liver. Up-regulates ACACA enzyme activity. Required for efficient lipid biosynthesis, including triacylglycerol, diacylglycerol and phospholipid. Involved in stabilization of microtubules. Q9NPA3 NM_021242 NP_067065.1

MID1IP1 Antibody, MIG12 Antibody, S14R Antibody, Spot 14-R Antibody, Spot 14-related protein Antibody, Protein STRAIT11499 Antibody, G12-like Antibody, MID1 interacting protein 1 Antibody, Mid1-interacting protein 1 Antibody, STRAIT11499 Antibody, THRSPL Antibody


Specifications

Target
Human MID1IP1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Bovine (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:1000)
  • ICC (1:100 - 1:1000)
  • Western blot (1:500 - 1:3000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
MID1IP1 antibody was raised against recombinant fragment corresponding to a region within amino acids 1 and 183 of MID1IP1 (SwissProt Q9NPA3). Percent identity by BLAST analysis: Human (100%); Bovine (93%).
Epitope
aa1-183
Specificity
Human MID1IP1
Usage
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).
Presentation
0.1 M Tris-glycine, pH 7.0, 10% glycerol, 0.01% Thimerosal
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded A549 xenograft using MID1IP1 antibody at 1:100 dilution.
IHC of paraffin-embedded A549 xenograft using MID1IP1 antibody at 1:100 dilution.

Immunocytochemistry

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa using MID1IP1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with alpha-tubulin antibody (Red) at 1:2000.
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa using MID1IP1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with alpha-tubulin antibody (Red) at 1:2000.

Western blot

Sample (30 ug whole cell lysate). A:293T, B: Hep G2 . 12% SDS PAGE. MID1IP1 antibody diluted at 1:1000
Sample (30 ug whole cell lysate). A:293T, B: Hep G2 . 12% SDS PAGE. MID1IP1 antibody diluted at 1:1000

Immunohistochemistry

IHC of paraffin-embedded A549 xenograft using MID1IP1 antibody at 1:100 dilution.
IHC of paraffin-embedded A549 xenograft using MID1IP1 antibody at 1:100 dilution.

Immunocytochemistry

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa using MID1IP1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with alpha-tubulin antibody (Red) at 1:2000.
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa using MID1IP1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with alpha-tubulin antibody (Red) at 1:2000.

Western blot

Sample (30 ug whole cell lysate). A:293T, B: Hep G2 . 12% SDS PAGE. MID1IP1 antibody diluted at 1:1000
Sample (30 ug whole cell lysate). A:293T, B: Hep G2 . 12% SDS PAGE. MID1IP1 antibody diluted at 1:1000

Immunohistochemistry

IHC of paraffin-embedded A549 xenograft using MID1IP1 antibody at 1:100 dilution.
IHC of paraffin-embedded A549 xenograft using MID1IP1 antibody at 1:100 dilution.

Immunocytochemistry

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa using MID1IP1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with alpha-tubulin antibody (Red) at 1:2000.
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa using MID1IP1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with alpha-tubulin antibody (Red) at 1:2000.

Western blot

Sample (30 ug whole cell lysate). A:293T, B: Hep G2 . 12% SDS PAGE. MID1IP1 antibody diluted at 1:1000
Sample (30 ug whole cell lysate). A:293T, B: Hep G2 . 12% SDS PAGE. MID1IP1 antibody diluted at 1:1000

Immunohistochemistry

IHC of paraffin-embedded A549 xenograft using MID1IP1 antibody at 1:100 dilution.
IHC of paraffin-embedded A549 xenograft using MID1IP1 antibody at 1:100 dilution.

Immunocytochemistry

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa using MID1IP1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with alpha-tubulin antibody (Red) at 1:2000.
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa using MID1IP1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with alpha-tubulin antibody (Red) at 1:2000.

Western blot

Sample (30 ug whole cell lysate). A:293T, B: Hep G2 . 12% SDS PAGE. MID1IP1 antibody diluted at 1:1000
Sample (30 ug whole cell lysate). A:293T, B: Hep G2 . 12% SDS PAGE. MID1IP1 antibody diluted at 1:1000


Requested From: 
Date Requested: 6/18/2018

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