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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-B5981-50 50 µl (1 mg/ml) $425 
UCHL1 / PGP9.5 Antibody - Anti-UCHL1 / PGP9.5 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
UCHL1 / PGP9.5 Antibody - Anti-UCHL1 / PGP9.5 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
UCHL1 / PGP9.5 Antibody - Western blot analysis of lysates from 293 cells, using PGP9.5 Antibody. The lane on the right is blocked with the synthesized peptide.
UCHL1 / PGP9.5 Antibody - Anti-UCHL1 / PGP9.5 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
UCHL1 / PGP9.5 Antibody - Anti-UCHL1 / PGP9.5 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
UCHL1 / PGP9.5 Antibody - Western blot analysis of lysates from 293 cells, using PGP9.5 Antibody. The lane on the right is blocked with the synthesized peptide.
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PathPlus™ UCHL1 / PGP9.5 Antibody aa171‑220 Polyclonal IHC,Peptide‑ELISA,WB LS‑B5981

PathPlus™ UCHL1 / PGP9.5 Antibody aa171‑220 Polyclonal IHC,Peptide‑ELISA,WB LS‑B5981

Note: This antibody replaces LS-C118557
Antibody:
Rabbit Polyclonal to Human UCHL1 / PGP9.5
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B5981-50
50 µl
1 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human UCHL1 / PGP9.5
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
UCHL1 is a deubiquitinating enzyme found in neurons and neuroendocrine cells. UCHL1 functions to promote neuronal differentiation and regulates autophagy. UCHL1 is also considered an oncogene; mice engineered to overexpress UCHL1 demonstrate a propensity for lymphogenesis and malignancy, and abnormal expression of UCHL1 is seen in some lung and endometrial cancers. It has also been implicated in a number of neurodegenerative disorders including Parkinson’s and Alzheimer’s disease. In immunohistochemistry of normal tissue, UCHL1 is positive in neurons in the central nervous system. It is also present in a number of other tissues and cell types including distal renal tubules, islets of Langerhans, spermatogonia in testis, Leydig cells, oocytes and melanocytes. Chemistry & Biology. 10 (9): 837–46. doi:10.1016/j.chembiol.2003.08.010. PMID: 14522054; Leukemia. 2010 Sep;24(9):1641-55, PMID: 20574456; J Exp Clin Cancer Res. 2018 Oct 25;37(1):258, PMID: 30359286; Int J Gynecol Cancer. 2018 May;28(4):675-683, PMID: 29489474
Target
Human UCHL1 / PGP9.5
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:200)
  • Western blot (1:500 - 1:1000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:5000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide from human PGP9.5. (AA Range: 171-220)
Epitope
aa171-220
Specificity
PGP9.5 Antibody detects endogenous levels of total PGP9.5 protein.
Usage
Western Blot: Predicted MW 24 kDa
Presentation
PBS (without Mg2+, Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Storage
Store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About UCHL1 / PGP9.5
P09936 NM_004181 NP_004172.2

Validation

UCHL1 / PGP9.5 Antibody - Anti-UCHL1 / PGP9.5 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-UCHL1 / PGP9.5 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-UCHL1 / PGP9.5 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

UCHL1 / PGP9.5 Antibody - Anti-UCHL1 / PGP9.5 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-UCHL1 / PGP9.5 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-UCHL1 / PGP9.5 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

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Requested From: United States
Date Requested: 12/12/2019