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Catalog Number Size Price
LS-C407821-10 10 µg $318 
LS-C407821-100 100 µg $470 
LCN2 / Lipocalin 2 / NGAL Antibody - IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody. Lipocalin 2 was detected in frozen section of mouse spleen tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Lipocalin 2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
LCN2 / Lipocalin 2 / NGAL Antibody - IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody. Lipocalin 2 was detected in frozen section of rat lung tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Lipocalin 2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
LCN2 / Lipocalin 2 / NGAL Antibody - IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody. Lipocalin 2 was detected in frozen section of rat spleen tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Lipocalin 2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
LCN2 / Lipocalin 2 / NGAL Antibody - IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody. Lipocalin 2 was detected in frozen section of mouse lung tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Lipocalin 2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
LCN2 / Lipocalin 2 / NGAL Antibody - Lipocalin 2 antibody IHC-paraffin. IHC(P): Mouse Spleen Tissue.
LCN2 / Lipocalin 2 / NGAL Antibody - Lipocalin 2 antibody IHC-paraffin. IHC(P): Rat Spleen Tissue.
LCN2 / Lipocalin 2 / NGAL Antibody - IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody. Lipocalin 2 was detected in paraffin-embedded section of Rat Spleen Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Lipocalin 2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
LCN2 / Lipocalin 2 / NGAL Antibody - Lipocalin 2 antibody Western blot. All lanes: Anti Lipocalin 2 at 0.5 ug/ml. Lane 1: Rat Spleen Tissue Lysate at 50 ug. Lane 2: Rat Lung Tissue Lysate at 50 ug. Predicted band size: 22 kD. Observed band size: 22 kD.
LCN2 / Lipocalin 2 / NGAL Antibody - IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody. Lipocalin 2 was detected in frozen section of mouse spleen tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Lipocalin 2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
LCN2 / Lipocalin 2 / NGAL Antibody - IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody. Lipocalin 2 was detected in frozen section of rat lung tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Lipocalin 2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
LCN2 / Lipocalin 2 / NGAL Antibody - IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody. Lipocalin 2 was detected in frozen section of rat spleen tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Lipocalin 2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
LCN2 / Lipocalin 2 / NGAL Antibody - IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody. Lipocalin 2 was detected in frozen section of mouse lung tissue . Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Lipocalin 2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
LCN2 / Lipocalin 2 / NGAL Antibody - Lipocalin 2 antibody IHC-paraffin. IHC(P): Mouse Spleen Tissue.
LCN2 / Lipocalin 2 / NGAL Antibody - Lipocalin 2 antibody IHC-paraffin. IHC(P): Rat Spleen Tissue.
LCN2 / Lipocalin 2 / NGAL Antibody - IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody. Lipocalin 2 was detected in paraffin-embedded section of Rat Spleen Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-Lipocalin 2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
LCN2 / Lipocalin 2 / NGAL Antibody - Lipocalin 2 antibody Western blot. All lanes: Anti Lipocalin 2 at 0.5 ug/ml. Lane 1: Rat Spleen Tissue Lysate at 50 ug. Lane 2: Rat Lung Tissue Lysate at 50 ug. Predicted band size: 22 kD. Observed band size: 22 kD.
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Polyclonal Rabbit anti‑Mouse LCN2 / Lipocalin 2 / NGAL Antibody (aa21‑200, IHC, WB) LS‑C407821

Polyclonal Rabbit anti‑Mouse LCN2 / Lipocalin 2 / NGAL Antibody (aa21‑200, IHC, WB) LS‑C407821

Antibody:
LCN2 / Lipocalin 2 / NGAL Rabbit anti-Mouse Polyclonal (aa21-200) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C407821-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
LCN2 / Lipocalin 2 / NGAL Rabbit anti-Mouse Polyclonal (aa21-200) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
NGAL antibody LS-C407821 is an unconjugated rabbit polyclonal antibody to NGAL (LCN2 / Lipocalin 2) (aa21-200) from mouse. It is reactive with mouse and rat. Validated for ELISA, IHC and WB.
Target
Mouse LCN2 / Lipocalin 2 / NGAL
Synonyms
LCN2 | 24p3 | HNL | Lipocalin 2 | Lipocalin-2 | MSFI | Siderocalin LCN2 | NGAL | p25 | Lipocalin 2 (oncogene 24p3) | Oncogene 24p3
Host
Rabbit
Reactivity
Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived mouse Lipocalin 2 recombinant protein (Position: Q21-N200). Mouse Lipocalin 2 shares 62 % and 81.1 % amino acid (aa) sequence identity with human and rat Lipocalin 2, respectively.
Epitope
aa21-200
Specificity
Detected in lung, spleen, uterus, vagina and epididymis. .
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
  • ELISA (0.1 - 0.5 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
IHC: Antigen retrieval by boiling the paraffin sections in 10 mM citrate buffer, pH6.0, for 20 minutes is required for the staining of formalin/paraffin sections.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About LCN2 / Lipocalin 2 / NGAL
P80188 NM_005564 NP_005555.2

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 5/23/2024