Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
MGMT antibody was raised against recombinant human MGMT (O6-methylguanine-DNA methyltransferase) expressed in E. coli.
Recognizes MGMT in human tissues, tumors, cells or xenograft lines. Weak cross-reactivity with MGMT from mouse, hamster and rat cells.
Suitable for use in Western Blot, Flow Cytometry, Immunohistochemistry and Immunoprecipitation and Immunofluorescence. Western Blot: 1:500 detects MGMT on 10 ug of Jurkat lysates. Immunohistochemistry: paraffin sections. Immunofluorescence (IC): HeLa cells. Positive control: Tonsil tissue, HeLa cells, CEM-CCRF cells.
0.02 M PBS, pH 7.6, 0.1% sodium azide.
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.