Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(tested or 100% immunogen sequence identity)
Western blot (1:500)
ELISA (1:10000 - 1:50000)
MGMT antibody was raised against purified recombinant human MGMT protein (~25kD) expressed in E. coli. Cellular Localization: cytoplasmic and nuclear.
Recognizes human MGMT.
Suitable for use in ELISA and Western Blot. Western Blot: 1:500. Detects ~25kD in liver and many tumor cells (Molt-4, Raji, HeLa, HT-292, etc). ELISA: 1:10000-1:50000 using 50-100 ng control peptide/well.
Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.