(tested or 100% immunogen sequence identity)
- Western blot (1:500)
- ELISA (1:10000 - 1:50000)
MGMT antibody was raised against purified recombinant human MGMT protein (~25kD) expressed in E. coli. Cellular Localization: cytoplasmic and nuclear.
Recognizes human MGMT.
Suitable for use in ELISA and Western Blot. Western Blot: 1:500. Detects ~25kD in liver and many tumor cells (Molt-4, Raji, HeLa, HT-292, etc). ELISA: 1:10000-1:50000 using 50-100 ng control peptide/well.
100 mM Tris, pH 7.5, 0.05% Sodium Azide, 40% Glycerol, 0.2% BSA
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.