Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
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Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal
Protein G purified
Applications
IHC - Paraffin, Western blot (0.5 - 1 µg/ml), Flow Cytometry
Specificity and Use
MGMT antibody was raised against recombinant human MGMT protein. Cellular Localization: Nuclear and cytoplasmic. MW of Antigen: 25kD. Epitope: aa8-221.
aa8-221
Recognizes human MGMT (O6-methylguanine-DNA methyltransferase). Species cross-reactivity: Weakly cross-reacts with mouse, rat and hamster.
Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Western Blot: 0.5-1 ug/ml for 2hrs at RT. Immunohistochemistry (Formalin/paraffin): 1:10-1:20 for 60 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. Positive control: Raji cells; Colon carcinoma.
Packaging
PBS, pH 7.4, 0.2% BSA, 0.1% sodium azide.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
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