Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(tested or 100% immunogen sequence identity)
Protein G purified
IHC - Paraffin
Western blot (0.5 - 1 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
MGMT antibody was raised against recombinant human MGMT protein. Cellular Localization: Nuclear and cytoplasmic. MW of Antigen: 25kD. Epitope: aa8-221.
Recognizes human MGMT (O6-methylguanine-DNA methyltransferase). Species cross-reactivity: Weakly cross-reacts with mouse, rat and hamster.
Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Western Blot: 0.5-1 ug/ml for 2hrs at RT. Immunohistochemistry (Formalin/paraffin): 1:10-1:20 for 60 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. Positive control: Raji cells; Colon carcinoma.
PBS, pH 7.4, 0.1% Sodium Azide, 0.2% BSA
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.