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MGMT antibody LS-C9009 is an unconjugated mouse monoclonal antibody to human MGMT. Validated for Flow, IHC and WB.
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Species:
Human, Mouse, Rat, Hamster
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Flow Cytometry
Species:
Human, Mouse, Rat, Hamster
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Monkey, Mouse, Rat, Bat, Dog, Guinea pig, Hamster, Horse, Pig
Applications:
Western blot
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot
Product Description
MGMT antibody LS-C9009 is an unconjugated mouse monoclonal antibody to human MGMT. Validated for Flow, IHC and WB.
About MGMT
Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
P16455
NM_002412
NP_002403.2
MGMT Antibody
Specifications
Target
Human MGMT
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
- IHC
- IHC - Paraffin
- Western blot (0.5 - 1 µg/ml)
- Flow Cytometry
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Immunogen
MGMT antibody was raised against recombinant human MGMT protein. Cellular Localization: Nuclear and cytoplasmic. MW of Antigen: 25kD. Epitope: aa8-221.
Epitope
aa8-221
Specificity
Recognizes human MGMT (O6-methylguanine-DNA methyltransferase). Species cross-reactivity: Weakly cross-reacts with mouse, rat and hamster.
Usage
Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Western Blot: 0.5-1 ug/ml for 2hrs at RT. Immunohistochemistry (Formalin/paraffin): 1:10-1:20 for 60 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. Positive control: Raji cells; Colon carcinoma.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide, 0.2% BSA
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.
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