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Anti-MGMT Antibody (aa8-221) LS-C9009

Ordering

Wt. Vol. Conc. Price
- 500 µl - $495
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular MGMT Antibodies

Anti-MGMT Antibody (clone MT 3.1) LS-C88207
Mouse Monoclonal [clone MT 3.1] (IgG1) to Human MGMT
Human, Mouse, Rat, Hamster
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-MGMT Antibody (clone MT 3.1, Biotin) LS-C88208
Mouse Monoclonal [clone MT 3.1] (IgG1) to Human MGMT
Human, Mouse, Rat, Hamster
IHC - Paraffin, Western blot
Biotin Conjugated
Immunohistochemistry Image
Anti-MGMT Antibody (aa156-182) LS-C100520
Rabbit Polyclonal to Human MGMT
Human
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal (IgG1) to Human MGMT
Human
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated

Details

Human MGMT
Mouse
Human (tested or 100% immunogen sequence identity)
IgG1 Monoclonal
Unconjugated
Protein G purified
Unmodified

Applications

  • IHC - Paraffin
  • Western blot (0.5 - 1 µg/ml)
  • Flow Cytometry

Specificity and Use

MGMT antibody was raised against recombinant human MGMT protein. Cellular Localization: Nuclear and cytoplasmic. MW of Antigen: 25kD. Epitope: aa8-221.
aa8-221
Recognizes human MGMT (O6-methylguanine-DNA methyltransferase). Species cross-reactivity: Weakly cross-reacts with mouse, rat and hamster.
Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Western Blot: 0.5-1 ug/ml for 2hrs at RT. Immunohistochemistry (Formalin/paraffin): 1:10-1:20 for 60 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. Positive control: Raji cells; Colon carcinoma.

Packaging

PBS, pH 7.4, 0.2% BSA, 0.1% sodium azide.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
For research use only.

About MGMT

P16455 NM_002412 NP_002403.2

MGMT Antibody

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

Requested From: United States
Date Requested: 12/5/2016

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