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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MGMT Antibody (aa8‑221) LS‑C9009
Antibody
MGMT antibody LS-C9009 is an unconjugated mouse monoclonal antibody to human MGMT. Validated for Flow, IHC and WB.
Catalog
Size
Price
LS-C9009-500
500 µl
Unavailable

Popular MGMT Products

Formalin-fixed, paraffin-embedded human colon carcinoma stained with peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells.  This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat, Hamster
Applications: IHC, IHC - Paraffin, Western blot
Formalin-fixed, paraffin-embedded human colon carcinoma stained with peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells.  This image was taken for the unmodified form of this product. Other forms have not been tested.
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MGMT antibody LS-C116198 Western blot of A549 cell lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Species: Human, Mouse, Rat
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Product Description

MGMT antibody LS-C9009 is an unconjugated mouse monoclonal antibody to human MGMT. Validated for Flow, IHC and WB.
About MGMT
Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. P16455 NM_002412 NP_002403.2

MGMT Antibody


Specifications

Target
Human MGMT
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot (0.5 - 1 µg/ml)
  • Flow Cytometry
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
MGMT antibody was raised against recombinant human MGMT protein. Cellular Localization: Nuclear and cytoplasmic. MW of Antigen: 25kD. Epitope: aa8-221.
Epitope
aa8-221
Specificity
Recognizes human MGMT (O6-methylguanine-DNA methyltransferase). Species cross-reactivity: Weakly cross-reacts with mouse, rat and hamster.
Usage
Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Western Blot: 0.5-1 ug/ml for 2hrs at RT. Immunohistochemistry (Formalin/paraffin): 1:10-1:20 for 60 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. Positive control: Raji cells; Colon carcinoma.
Presentation
PBS, pH 7.4, 0.2% BSA, 0.1% sodium azide.
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/24/2018

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