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Antibodies > MGMT
MGMT Antibody (aa8‑221) LS‑C9009
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MGMT antibody LS-C9009 is an unconjugated mouse monoclonal antibody to human MGMT. Validated for Flow, IHC and WB.
Antibody
Catalog
Size
Price
LS-C9009-500
500 µl
$635
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Product Description

MGMT antibody LS-C9009 is an unconjugated mouse monoclonal antibody to human MGMT. Validated for Flow, IHC and WB.
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Specifications

Target
Human MGMT
Synonyms
MGMT
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot (0.5 - 1 µg/ml)
  • Flow Cytometry
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
MGMT antibody was raised against recombinant human MGMT protein. Cellular Localization: Nuclear and cytoplasmic. MW of Antigen: 25kD. Epitope: aa8-221.
Epitope
aa8-221
Specificity
Recognizes human MGMT (O6-methylguanine-DNA methyltransferase). Species cross-reactivity: Weakly cross-reacts with mouse, rat and hamster.
Usage
Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Western Blot: 0.5-1 ug/ml for 2hrs at RT. Immunohistochemistry (Formalin/paraffin): 1:10-1:20 for 60 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. Positive control: Raji cells; Colon carcinoma.
Presentation
PBS, pH 7.4, 0.1% Sodium Azide, 0.2% BSA
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
About MGMT
Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. P16455 NM_002412 NP_002403.2

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Requested From: United States
Date Requested: 11/15/2018
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