ERK1 antibody LS-C16352 is an unconjugated rabbit polyclonal antibody to ERK1 (MAPK3) from human, mouse, rat and other species. Validated for ELISA, Flow, ICC, IF, IP and WB.
Human MAPK3 / ERK1
Human, Mouse, Rat, Hamster, Chicken
(tested or 100% immunogen sequence identity)
- Western blot (1:1000)
- Immunoprecipitation (1:50)
- Flow Cytometry (1:100)
MAPK3 / ERK1 antibody was raised against synthetic phosphopeptide corresponding to residues around Thr202/Tyr204 of human p44 MAP kinase (KLH).
Detects endogenous levels of human p42 and p44 MAP kinase (Erk1 and Erk2) only when phosphorylated either individually or dually at Thr202 and Thr204 of Erk1 (Thr185 an Tyr187 of Erk2). Does not cross-react with the corresponding phosphorylated residues of either JNK/SAPK or p38 MAP kinase, or non-phosphorylated Erk1/2. Species cross-reactivity: chicken, hamster, mouse, rat, zebrafish. bovine and monkey.
Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunofluorescence and Immunocytochemistry. Western Blot: 1:1000. Incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:50. Immunofluorescence (IF-IC): 1:1000. Flow Cytometry: 1:100.
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 50% Glycerol, No preservatives added
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.