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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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MAPK3 / ERK1 Antibody (phospho‑Thr202/Tyr204) LS‑C16352
ERK1 antibody LS-C16352 is an unconjugated rabbit polyclonal antibody to ERK1 (MAPK3) from human, mouse, rat and other species. Validated for ELISA, Flow, ICC, IF, IP and WB.
Antibody
Catalog
Size
Price
LS-C16352-200
200 µl
$575

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Product Description

ERK1 antibody LS-C16352 is an unconjugated rabbit polyclonal antibody to ERK1 (MAPK3) from human, mouse, rat and other species. Validated for ELISA, Flow, ICC, IF, IP and WB.
About MAPK3 / ERK1
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. P27361 X60188 CAA42744.1

MAPK3 Antibody, ERK-1 Antibody, ERT2 Antibody, HUMKER1A Antibody, HS44KDAP Antibody, MAP kinase 1 Antibody, MAP kinase isoform p44 Antibody, MAPK 1 Antibody, MAPK 3 Antibody, p44 Antibody, p44MAPK Antibody, p44-MAPK Antibody, p44ERK1 Antibody, Insulin-stimulated MAP2 kinase Antibody, MAP kinase 3 Antibody, p44-ERK1 Antibody, ERK1 Antibody, PRKM3 Antibody


Specifications

Target
Human MAPK3 / ERK1
Host
Rabbit
Reactivity
Human, Mouse, Rat, Hamster, Chicken (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • ICC
  • Immunofluorescence
  • Western blot (1:1000)
  • Immunoprecipitation (1:50)
  • Flow Cytometry (1:100)
  • ELISA
Immunogen
MAPK3 / ERK1 antibody was raised against synthetic phosphopeptide corresponding to residues around Thr202/Tyr204 of human p44 MAP kinase (KLH).
Epitope
pThr202/Tyr204
Specificity
Detects endogenous levels of human p42 and p44 MAP kinase (Erk1 and Erk2) only when phosphorylated either individually or dually at Thr202 and Thr204 of Erk1 (Thr185 an Tyr187 of Erk2). Does not cross-react with the corresponding phosphorylated residues of either JNK/SAPK or p38 MAP kinase, or non-phosphorylated Erk1/2. Species cross-reactivity: chicken, hamster, mouse, rat, zebrafish. bovine and monkey.
Usage
Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunofluorescence and Immunocytochemistry. Western Blot: 1:1000. Incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4?C with gentle shaking, overnight. Immunoprecipitation: 1:50. Immunofluorescence (IF-IC): 1:1000. Flow Cytometry: 1:100.
Presentation
10 mM HEPES, pH 7.5, 150 mM NaCl, 0.1 mg/ml BSA, 50% Glycerol, No preservatives added
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: United States
Date Requested: 8/19/2018

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