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| Catalog Number | Size | Price |
|---|---|---|
| LS-C16384-100 | 100 µg | $947 |
Monoclonal Mouse anti‑Human MAPK3 / ERK1 Antibody (Biotin, phospho‑Thr202/Thr204, WB) LS‑C16384
Monoclonal Mouse anti‑Human MAPK3 / ERK1 Antibody (Biotin, phospho‑Thr202/Thr204, WB) LS‑C16384
Antibody:
MAPK3 / ERK1 Mouse anti-Human Monoclonal (pThr202/Thr204) (Biotin) Antibody
Application:
ICC, WB, IP, ELISA
Reactivity:
Human, Mouse, Rat, Dog
Format:
Biotin, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
MAPK3 / ERK1 Mouse anti-Human Monoclonal (pThr202/Thr204) (Biotin) Antibody
Application:
ICC, WB, IP, ELISA
Reactivity:
Human, Mouse, Rat, Dog
Format:
Biotin, Unmodified
Specifications
Description
ERK1 antibody LS-C16384 is a biotin-conjugated mouse monoclonal antibody to ERK1 (MAPK3) (pThr202/Thr204) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, ICC, IP and WB.
Target
Human MAPK3 / ERK1
Synonyms
MAPK3 | ERK-1 | ERT2 | HUMKER1A | HS44KDAP | MAP kinase 1 | MAP kinase isoform p44 | MAPK 1 | MAPK 3 | p44 | p44MAPK | p44-MAPK | p44ERK1 | MAP kinase 3 | p44-ERK1 | ERK1 | PRKM3
Host
Mouse
Reactivity
Human, Mouse, Rat, Dog
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Conjugations
Biotin
Purification
Gel filtration
Modifications
Unmodified
Immunogen
Synthetic peptide corresponding to the FLpTEpYVA motif of phosphorylated ERK1/2 (MAPK2/3). Conjugated to KLH.
Epitope
pThr202/Thr204
Specificity
Recognizes the FLpTEpYVA motif of activated human ERK1 and ERK2. Requires phosphorylation both at the threonine and the tyrosine site and does not cross-react with the non-phosphorylated form of the protein. Does not cross-react with activated JNK or p38. Species cross-reactivity: mouse, rat, and canine.
Applications
- ICC (1 - 10 µg/ml)
- Western blot (0.5 µg/ml)
- Immunoprecipitation (1 µg/10E6 cells)
- ELISA (0.05 µg/ml)
Usage
IP: Pervanadate treated A431 or HepG2 cells. WB: For ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
Presentation
PBS, 0.09% Sodium Azide, 40% Glycerol, Sucrose
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About MAPK3 / ERK1
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Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 9/19/2024
Date Requested: 9/19/2024
