Human MAPK3 / ERK1

Rabbit

Human (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

Synthetic peptide from human ERK1/ERK2.

pThr185/Tyr187

Synthetic phosphopeptide derived from the region of human ERK1&2 that contains threonine 202/185 and tyrosine 204/187. This region is

• Western blot

The antibody Peptide Competition. Extracts prepared from PC12 cells left unstimulated or stimulated with sorbitol. were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to. PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4?C, then. were incubated with ERK1&2 [pTpY185/187] antibody for two hours at room. temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide., the non-phosphopeptide corresponding to the immunogen, a generic. phosphothreonine-containing peptide, a generic phosphotyrosine-containing. peptide, or, the phosphopeptide immunogen. After washing, membranes were. incubated with goat F (ab')2 anti-rabbit IgG HRP conjugate (Cat. # ALI4404) and. bands were detected using the Pierce SuperSignal method. The data show that only the peptide corresponding to ERK1&2 [pTpY185/187] blocks. the antibody signal, thereby demonstrating the specificity of the antibody. The data. also show the induction of phosphorylation by the addition of sorbitol. y has been used for Western blot and immunostaining applications. Positive control: PC12 cells +/- Sorbitol.

1 mg/ml BSA (IgG, Protease free), PBS (without Mg2+, Ca2+), pH 7.2, 0.1% Sodium Azide, 50% Glycerol

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P27361 NCBI: X60188 CAA42744.1