LSBio The Immunohistochemistry Antibody Company
Products
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA Kits
  • All Kits
  • Sandwich ELISA Kits
  • Competitive EIA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Direct ELISA Kits
  • Functional ELISA Kits
  • Phospho-Specific ELISA Kits
  • Custom ELISA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Immunohistochemistry
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
  • Other
  • Blocking Peptides
Services
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question? CustomerSupport@LSBio.com
Resources
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Our Rewards Program
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Publications
  • Secure Logins
  • Login to Our Secure FTP Site
  • Login to Our IHC Database
Distributors
Contact Us

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Quick Order ▾
View Cart

Anti-MAPK3 / ERK1 Antibody (phospho-Thr185/Tyr187) LS-C15048

Ordering

Wt. Vol. Conc. Price
- 10 blot - $515
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular MAPK3 / ERK1 Antibodies

Anti-MAPK3 / ERK1 Antibody (Internal) IHC-plus™ LS-A2877
Rabbit Polyclonal to Human MAPK3 / ERK1
Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig
IHC - Paraffin
Unconjugated
Immunohistochemistry Image
Anti-MAPK3 / ERK1 Antibody (phospho-Thr202/Tyr204) LS-C16352
Rabbit Polyclonal (IgG) to Human MAPK3 / ERK1
Human, Mouse, Rat, Hamster, Chicken
ICC, Immunofluorescence, Western blot, Immunoprecipitation, Flow Cytometry, ELISA
Unconjugated
Anti-MAPK3 / ERK1 Antibody (C-Terminus) LS-C209857
Rabbit Polyclonal (IgG) to Zebrafish MAPK3 / ERK1
Zebrafish
Western blot, ELISA
Unconjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human MAPK3 / ERK1
Human
Western blot
Unconjugated

Details

Human MAPK3 / ERK1
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

Western blot

Specificity and Use

MAPK3 / ERK1 antibody was raised against synthetic peptide from human ERK1/ERK2.
pThr185/Tyr187
Synthetic phosphopeptide derived from the region of human ERK1&2 that contains threonine 202/185 and tyrosine 204/187. This region is
The antibody Peptide Competition. Extracts prepared from PC12 cells left unstimulated or stimulated with sorbitol. were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to. PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4?C, then. were incubated with ERK1&2 [pTpY185/187] antibody for two hours at room. temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide., the non-phosphopeptide corresponding to the immunogen, a generic. phosphothreonine-containing peptide, a generic phosphotyrosine-containing. peptide, or, the phosphopeptide immunogen. After washing, membranes were. incubated with goat F (ab')2 anti-rabbit IgG HRP conjugate (Cat. # ALI4404) and. bands were detected using the Pierce SuperSignal method. The data show that only the peptide corresponding to ERK1&2 [pTpY185/187] blocks. the antibody signal, thereby demonstrating the specificity of the antibody. The data. also show the induction of phosphorylation by the addition of sorbitol. y has been used for Western blot and immunostaining applications. Positive control: PC12 cells +/- Sorbitol.

Packaging

PBS (without Mg2+, Ca2+), 1 mg/ml BSA (IgG, protease free), pH 7.2, 0.1% sodium azide, 50% glycerol.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About MAPK3 / ERK1

P27361 X60188 CAA42744.1

MAPK3 Antibody, ERK-1 Antibody, ERT2 Antibody, HUMKER1A Antibody, HS44KDAP Antibody, MAP kinase 1 Antibody, MAP kinase isoform p44 Antibody, MAPK 1 Antibody, MAPK 3 Antibody, p44 Antibody, p44MAPK Antibody, p44-MAPK Antibody, p44ERK1 Antibody, Insulin-stimulated MAP2 kinase Antibody, MAP kinase 3 Antibody, p44-ERK1 Antibody, ERK1 Antibody, PRKM3 Antibody

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF.

Requested From: United States
Date Requested: 12/7/2016

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn
Copyright © 2016 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy
Catalog Number