Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rat, Human, Mouse, Bovine, Dog, Chicken (tested or 100% immunogen sequence identity)
IHC (1:250 - 1:500), Western blot (1:1000)
Specificity and Use
MAPK14 / p38 antibody was raised against phosphopeptide corresponding to amino acid residues surrounding the phosphoThr180 and phospho-Tyr182 of rat p38 MAPK.
Specific for the ~39k p38 MAPK protein phosphorylated at Thr180/Tyr182. Immunolabeling is blocked by preadsorption of the antibody with the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.
10 mM sodium HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/ml BSA, 50% glycerol.
MAP kinase p38 alpha, a MAPK type protein kinase, is an environmental stress- and inflammatory cytokine-induced kinase that is associated with the regulation of gene expression, cell proliferation and cell death. p38alpha is phosphorylated by MEK3/MAP2K3 or MKK6/MAP2K6.
Western blot of HeLa cell lysates that had been treated with UV or untreated (Control) showing specific immunolabeling of the ~39k p38 MAPK protein phosphorylated at Thr180 and Tyr182.
Western blot of C-6 glioma cell lysates in the presence of Anisomycin showing specific immunolabeling of the ~39k p38 protein phosphorylated at Thr180 and Tyr182 (Anisomycin). The immunolabeling is absent without Anisomycin (Control).
Requested From: United States
Date Requested: 9/29/2016