Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
The antibody pair provided in this kit recognizes human and mouse Phospho-P38 alpha (pThr180/pTyr182).
LS-F545 is a 96-well enzyme-linked immunosorbent assay (ELISA) for the Semi-Quantitative detection of Human MAPK14 / p38 in samples of Cell Lysates and Tissue Homogenates. It is based upon a Sandwich Phosphorylation assay principle.
All LSBio Custom kits have been predesigned and all necessary components, including antibodies and standards have been identified. Upon receiving a custom kit order, the kit will be assembled and quality control tested before being shipped out. Kit assembly and testing typically takes 4 to 6 weeks. In most cases the final kit is based on the Sandwich assay principle, with a few being Competitive EIA based. Specifications such as Range, Sensitivity, and Precision are defined upon completion. In the event that the custom kit cannot be successfully developed with 6 weeks of the order date, the customer will be notified and the order canceled at no cost.
Cell Lysates, Tissue Homogenates
Colorimetric - 450nm
Due to their limited shelf life, LSBio ELISA kits are not typically stocked as finished goods. Upon receipt of an order each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Range, Sensitivity, and Precision. In the event of a significant change the order would be confirmed with the customer before shipping. ELISA kit lot numbers reflect the date of final assembly and testing for each specific kit rather than a bulk manufactured lot. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation.
Microplate - Item A
Wash Buffer Concentrate (20x) - Item B
Assay Diluent (Item E)
Biotinylated Detection Antibody
HRP-conjugated Anti-rabbit IgG - Item D-1
Stop Solution - Item I
Cell Lysate Buffer - Item J
Positive Control HelaA003-1 - Item K
Adhesive Plate Sealers
Upon receipt, the kit should be stored at –20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at –20 °C. Item D-1 store at 2-8 oC for up to one month (store at -20 oC for up to 6 months, avoid repeated freeze-thaw cycles). Reconstituted Positive Control (Item K) should be stored at -70 °C.
MAP kinase p38 alpha, a MAPK type protein kinase, is an environmental stress- and inflammatory cytokine-induced kinase that is associated with the regulation of gene expression, cell proliferation and cell death. p38alpha is phosphorylated by MEK3/MAP2K3 or MKK6/MAP2K6.