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Anti-IL1A / IL-1 Alpha Antibody LS-C104666

Note: This antibody replaces LS-C7681, LS-C54103, LS-C42229

Ordering

Wt. Vol. Conc. Price
50 µg - - $285
100 µg - - $325
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular IL1A / IL-1 Alpha Antibodies

Anti-IL1A / IL-1 Alpha Antibody IHC-plus™ LS-B1581
Mouse Monoclonal (IgG2a) to Human IL1A / IL-1 Alpha
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-IL1A / IL-1 Alpha Antibody LS-C104666
Rabbit Polyclonal to Mouse IL1A / IL-1 Alpha
Mouse
IHC, Western blot, ELISA, Neutralization
Unconjugated
Immunohistochemistry Image
Anti-IL1A / IL-1 Alpha Antibody (aa256-270) LS-C172276
Rabbit Polyclonal to Mouse IL1A / IL-1 Alpha
Mouse
IHC - Paraffin, Western blot
Unconjugated

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Mouse IL1A / IL-1 Alpha
Mouse
IHC, Western blot, ELISA, Neutralization (applications tested for the base form of this product only)
Unconjugated

Details

Mouse IL1A / IL-1 Alpha
Rabbit
Mouse (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin.
Immunoaffinity purified
Unmodified

Applications

  • IHC
  • Western blot
  • ELISA (0.5 - 2 µg/ml)
  • Neutralization (0.1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

IL1A / IL-1 Alpha antibody was raised against e.coli derived recombinant Murine IL-1alpha.
Mouse IL-1alpha
Immunohistochemistry: This antibody stained colchicine injected mouse brain (including the hippocampal fissure) tissue. The primary antibody was incubated at 1 mg/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of mIL-1alpha (0.05 ng/ml), a concentration of 0 3-0.05 ug/ml of this antibody is required. ELISA: To detect mIL-1alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine IL-1alpha (LS-C104815) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-1alpha. Western Blot: To detect mIL-1alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1alpha is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Packaging

Lyophilized from PBS, pH 7.2
Sterile water
Store Lyophilized at room temperature up to 1 month; Reconstituted for up to two weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
For research use only.

About IL1A / IL-1 Alpha

P01583 NM_000575 NP_000566.3

IL1A Antibody, Hematopoietin-1 Antibody, IL-1 alpha Antibody, IL1 Antibody, IL1-ALPHA Antibody, IL1 Alpha Antibody, Interleukin-1 alpha Antibody, IL-1A Antibody, IL1F1 Antibody, Pro-interleukin-1-alpha Antibody, Preinterleukin 1 alpha Antibody, Interleukin 1, alpha Antibody

IL1A / IL-1 Alpha is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.

Immunohistochemistry

Immunohistochemistry
IHC of IL1A / IL1 Alpha antibody LS-C104666. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

Immunohistochemistry
IHC of IL1A / IL1 Alpha antibody LS-C104666. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Western Blot (reducing) of IL1A / IL1 Alpha antibody LS-C104666. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
Western Blot (non-reducing) of IL1A / IL1 Alpha antibody LS-C104666. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Sandwich ELISA of IL1A / IL1 Alpha antibody LS-C104666. This image was taken for the unconjugated form of this product. Other forms have not been tested.
 

Requested From: United States
Date Requested: 12/3/2016

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