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Anti-IL1A / IL-1 Alpha Antibody LS-C7680

Ordering

Wt. Vol. Conc. Price
1000 µg - 1 mg/ml $635
Inquire for larger quantities

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Rabbit Polyclonal (IgG) to Human IL1A / IL-1 Alpha
Human
IHC - Paraffin, IHC - Frozen, Western blot, Flow Cytometry, ELISA, Neutralization
Unconjugated

Details

Human IL1A / IL-1 Alpha
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Ion exchange chromatography

Applications

IHC - Paraffin (1:200), IHC - Frozen (1:200), Western blot (1:100), Flow Cytometry, ELISA (1:200), Neutralization (1:100)

Specificity and Use

IL1A / IL-1 Alpha antibody was raised against recombinant human IL-1a produced in E. coli.
Primarily directed against the 17000 MW human IL-1a and is useful in determining its presence in various assays. Also detects primate IL-1a in the same formats using similar dilutions. Does not recognize human IL-1b or Mouse or Rabbit IL-1a. Recognizes both the mature 17000 MW IL-1a as well as the 31000 MW IL-1a precursor in either non-denatured (native) or denatured samples. Unlike the IL-1b precursor, the native precursor of IL-1a is recognized by the antibody produced to the 17000 MW form.
Suitable for use in Neutralizations, ELISA, Immunohistochemistry, Flow Cytometry and Western Blot. Western Blot: 1:100 to 1:200, use HRP Goat-anti-Rabbit IgG [H&L] and TMB as a substrate to detect IL-1a from supernatants or lysates of 2x1E6 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1% (v/v) human serum plus 10 ng/mL E. coli LPS. Immunohistochemistry (Paraffin/Frozen): 1:200. ELISA: 1:200 to 1:1000, using HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) and ABTS as a substrate for 30 minutes at RT. For use in ELISA formats, this antibody is best used as the second antibody in combination with a monoclonal antibody as a capture antibody. Neutralization: Incubate the sample with a 1:100. dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is. recommended. Flow Cytometry: Caution should be exhibited as the F ( c) domain of the rabbit IgG molecule may interact with cells non-specifically.

Packaging

PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added.
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About IL1A / IL-1 Alpha

P01583 NM_000575 NP_000566.3

IL1A Antibody, Hematopoietin-1 Antibody, IL-1 alpha Antibody, IL1 Antibody, IL1-ALPHA Antibody, IL1 Alpha Antibody, Interleukin-1 alpha Antibody, IL-1A Antibody, IL1F1 Antibody, Pro-interleukin-1-alpha Antibody, Preinterleukin 1 alpha Antibody, Interleukin 1, alpha Antibody

IL1A / IL-1 Alpha is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.

Requested From: United States
Date Requested: 9/28/2016

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