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Anti-Histone H2B Antibody (Acetyl-Lys12/Lys15/Lys20) LS-C24294

Catalog Size Price
LS-C24294-100 100 µg Unavailable

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100% Guaranteed
Rabbit Polyclonal (IgG) to Human Histone H2B
Human, Mouse, Rat, Bovine
Western blot (applications tested for the base form of this product only)


Human Histone H2B
Human, Mouse, Rat, Bovine (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Protein A purified


  • Western blot
  • (applications tested for the base form of this product only)

Specificity and Use

Histone H2B antibody was raised against kLH-conjugated, synthetic peptide (. S[AcK]SAPAPK[AcK]GS[AcK]KAIT[AcK]AQ.) in which AcK corresponds to acetyl-lysine at residues 5, 12, 15 and 20 of human Histone H2B. The immunizing sequence has 24 out of 26 identical amino acids in mouse, rat and bovine.
Recognizes acetylated Histone H2B, Mr ~15kD. Does not recognize acetylated Histone H2A or unacetylated recombinant Histone H2B protein. Species cross-reactivity: Human. Predicted to cross-react with mouse, rat and bovine based on sequence homology.
Suitable for use in Western Blot. Western Blot Analysis: 1:40000-1:80000 dilution of this lot detected acetylated Histone H2B in sodium butyrate treated, acid extracted proteins from HeLa cells. Unacetylated recombinant Histone H2B was not detected.


0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05% sodium azide, 30% glycerol.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About Histone H2B

Histone H2B is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2B is involved with the structure of the nucleosomes of the 'beads on a string' structure.

Requested From: 
Date Requested: 3/23/2018

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