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IHCPlus™ Histone H2B Antibody aa111‑125 Polyclonal IHC,WB LS‑B2035

Catalog Number Size Price
LS-B2035-50 50 µg (1 mg/ml) $425 
Anti-Histone H2B antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Western blot of Histone H2B in human PBMC lysate with antibody.
Western blot of Histone H2B using antibody at 1:250 in nuclear and cytoplasmic fractionsumade from Drosophila head extracts.
Western blot of fractionated Cos-7 cells using antibody anti-Histone H2B.? Cos-7 cells were fractionated into nuclear & cytosolic/membrane fractions. Approximately ~15 ug of each fraction was?separated on SDS PAGE.?? Lane 1, Nuclear fraction.ulane
Anti-Histone H2B antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Western blot of Histone H2B in human PBMC lysate with antibody.
Western blot of Histone H2B using antibody at 1:250 in nuclear and cytoplasmic fractionsumade from Drosophila head extracts.
Western blot of fractionated Cos-7 cells using antibody anti-Histone H2B.? Cos-7 cells were fractionated into nuclear & cytosolic/membrane fractions. Approximately ~15 ug of each fraction was?separated on SDS PAGE.?? Lane 1, Nuclear fraction.ulane
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IHCPlus™ Histone H2B Antibody aa111‑125 Polyclonal IHC,WB LS‑B2035

Note: This antibody replaces LS-C696
Antibody:
Rabbit Polyclonal to Human Histone H2B
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat, Bovine, Chicken, Zebrafish, Drosophila, Insect
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B2035-50
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human Histone H2B
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat, Bovine, Chicken, Zebrafish, Drosophila, Insect
Format:
Unconjugated, Unmodified

Specifications

Description
Histone H2B antibody LS-B2035 is an unconjugated rabbit polyclonal antibody to Histone H2B from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human Histone H2B
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Chicken, Zebrafish, Drosophila, Insect (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 µg/ml)
  • Western blot (2 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
This antibody was generated by immunizing rabbits with a synthetic peptide corresponding to amino acid residues 111-125 AVSEGTKAVTKYTSSof human Histone H2B; GenBank no. CAI19747.1. This peptide sequence is 100% conserved between multiple species including human, mouse, rat, dog, chimpanzee, cow, chicken, duck, zebrafish, and drosophila. Percent identity by BLAST analysis: Human, Orangutan, Mouse, Rat, Bovine, Chicken, Stickleback, Pufferfish, Zebrafish, Drosophila, Mosquito, Nematode (100%).
Epitope
aa111-125
Specificity
A 15 kD band is observed. This is routinely quality controlled in human lysates.
Usage
Immunohistochemistry: LS-B2035 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2035 was determined to be 5 ug/ml.
Presentation
PBS, 0.05% Sodium Azide, 0.05% BSA
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

Validation

Anti-Histone H2B antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC Validated
Anti-Histone H2B antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-Histone H2B antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Publications (1)

Transcriptional enhancement of Smn levels in motoneurons is crucial for proper axon morphology in zebrafish. Spiró Z, Koh A, Tay S, See K, Winkler C. Scientific reports. 2016 6:27470. (IF; Zebrafish)

Reviews (0)

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Requested From: United States
Date Requested: 9/23/2019