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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Histone H2B

Histone H2B is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2B is involved with the structure of the nucleosomes of the 'beads on a string' structure.

Histone H2B Target Details

Target Name: Histone H2B

Publications (2)

1
Epigenetic modifications induced by RGC-32 in colon cancer. Vlaicu SI, Tegla CA, Cudrici CD, Fosbrink M, Nguyen V, Azimzadeh P, Rus V, Chen H, Mircea PA, Shamsuddin A, Rus H. Experimental and molecular pathology. 2010 88:67-76. (WB) [PubMed:19883641] [PMC:PMC2815209]
2
Transcriptional enhancement of Smn levels in motoneurons is crucial for proper axon morphology in zebrafish. Spiró Z, Koh A, Tay S, See K, Winkler C. Scientific reports. 2016 6:27470. (IF; Zebrafish) [Full Text Article] [PubMed:27273160] [PMC:PMC4895340] Related Antibodies: LS-B2035.

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Target
Histone H2B (145)
Reactivity
Human (130)
Mouse (91)
Rat (64)
Bat (2)
Bovine (25)
Cairina moschata (4)
Chicken (11)
Dog (6)
Drosophila (1)
Hamster (3)
Horse (2)
Insect (1)
Monkey (13)
Pig (2)
Rabbit (2)
Xenopus (7)
Zebrafish (4)
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IHC (41)
IHC-P (25)
WB (137)
ChrIP (2)
DB (2)
ELISA (51)
ICC (11)
IF (30)
IP (14)
Peptide-ELISA (10)
Host
rabbit (130)
mouse (5)
sheep (10)
Isotype
IgG (113)
IgG2b (1)
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monoclonal mc (7)
polyclonal pc (138)
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FITC Conjugated (8)
HRP Conjugated (8)
PE Conjugated (6)
Unconjugated (103)
Epitope
aa1-30 (14)
aa26-52 (7)
1-80 aa (5)
Acetyl-Lys5 (5)
Acetyl-Lys12 (4)
N-Terminus (4)
aa2-93 (4)
C-Terminus (3)
aa1-50 (3)
10-90 aa (2)
Acetyl-Lys15 (2)
aa111-125 (2)
pSer14 (2)
10-90 aa, N-terminal (1)
Acetyl-Lys12/Lys15/Lys20 (1)
Acetyl-Lys20 (1)
Internal (1)
Lys24 (1)
Lys5 (1)
Pro8 (1)
Ubiquityl-Lys120 (1)
aa10-59 (1)
aa11-60 (1)
aa118-126 (1)
aa24-73 (1)
acetylation site of K126. (1)
pSer32 (1)
Publications
No (144)
Yes (1)
Showing 1-25 of 145 results

IHC-plus™ Histone H2B Antibody (aa111-125) LS-B2035
Publications (1)
Publications (1)
Rabbit Polyclonal (IgG) to Human Histone H2B
Chicken, Zebrafish, Insect, Drosophila, Bovine, Mouse, Rat, Human
IHC, IHC-P, WB
Unconjugated
50µg 1mg/ml  $425.00
Anti-Histone H2B antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Western blot of Histone H2B in human PBMC lysate with antibody.

IHC-plus™ Histone H2B Antibody (Acetyl-Lys12) LS-B2446
Rabbit Polyclonal (IgG) to Human Histone H2B
Chicken, Mouse, Rat, Human
IHC, IHC-P, WB
Unconjugated
50µl  $425.00
Anti-Histone H2B antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

IHC-plus™ Histone H2B Antibody (aa1-50) LS-B7677
Rabbit Polyclonal (IgG) to Human Histone H2B
Mouse, Human
IF, IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50µl 1mg/ml  $425.00
Anti-Histone H2B antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Immunofluorescence analysis of HeLa cells, using Histone H2B Antibody. The picture on the right is blocked with the synthesized peptide.

Histone H2B Antibody (N-Terminus) LS-C48570
Rabbit Polyclonal to Human Histone H2B
Mouse, Rat, Human
IP, WB
Unconjugated
30µg  $245.00; 100µg 200µl  $425.00
Western blot of Histone H2B expression with Jurkat cell lysate.

IHC-plus™ Histone H2B Antibody (aa10-59) LS-B7716
Rabbit Polyclonal (IgG) to Human Histone H2B
Mouse, Human
IF, IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50µl 1mg/ml  $425.00
Anti-Histone H2B antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.
Immunofluorescence analysis of HeLa cells, using Histone H2B Antibody. The picture on the right is blocked with the synthesized peptide.

IHC-plus™ Histone H2B Antibody (aa1-50) LS-B7712
Rabbit Polyclonal (IgG) to Human Histone H2B
Mouse, Human
IF, IHC, IHC-P, Peptide-ELISA
Unconjugated
50µl 1mg/ml  $425.00
Anti-Histone H2B antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.
Immunofluorescence analysis of HeLa cells, using Histone H2B Antibody. The picture on the right is blocked with the synthesized peptide.

Histone H2B Antibody (FITC) LS-C210869
Rabbit Polyclonal (IgG) to Human Histone H2B
Human
ELISA, IHC, WB
FITC Conjugated
50µg  $285.00; 100µg 0.25mg/ml  $385.00

Histone H2B Antibody LS-C24291
Sheep Polyclonal (IgG) to Bovine Histone H2B
Bovine
IF, IP, WB
Unconjugated
250µg  $680.00

Histone H2B Antibody LS-C203156
Rabbit Monoclonal (IgG)
ELISA, WB
Unconjugated
40µg 80µl 0.5mg/ml  $245.00
Western blot of HeLa cell lysates using 1 ug/ml Rabbit Anti-Histone H2B (Acetyl-K20) Polyclonal Antibody. The signal was developed with IRDye 800 Conjugated Goat Anti-Rabbit IgG. Predicted molecular weight: 14 KD. Observed molecular weight: 14 KD.
Western blot of cell and tissue lysates using 1 ug/ml Rabbit Anti-Histone H2B (Acetyl-K20) Polyclonal Antibody. The signal was developed with IRDye 800 Conjugated Goat Anti-Rabbit IgG. Predicted molecular weight: 14 KD. Observed molecular weight: 14 KD.

IHC-plus™ Histone H2B Antibody (aa11-60) LS-B7717
Rabbit Polyclonal (IgG) to Human Histone H2B
Mouse, Human
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50µl 1mg/ml  $425.00
Anti-Histone H2B antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10 ug/ml.
Western blot analysis of lysates from Raw264.7 cells, treated with TSA 400nM 24h, using Histone H2B Antibody. The lane on the right is blocked with the synthesized peptide.

Histone H2B Antibody (Acetyl-Lys12) LS-C356084
Rabbit Polyclonal to Human Histone H2B
Chicken, Bovine, Mouse, Dog, Human, Monkey
ICC, IF, IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $265.00
Immunohistochemical analysis of Histone H2B (AcK12) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of Histone H2B (AcK12) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Histone H2B Antibody (Acetyl-Lys15) LS-C356085
Rabbit Polyclonal to Human Histone H2B
Mouse, Dog, Bovine, Rat, Human, Monkey
ICC, IF, IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $265.00
Immunohistochemical analysis of Histone H2B (AcK15) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of Histone H2B (AcK15) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Histone H2B Antibody (N-Terminus) LS-C353496
Rabbit Polyclonal to Human Histone H2B
Chicken, Zebrafish, Mouse, Bovine, Human, Monkey
ChrIP, ICC, IF, IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $245.00
Immunohistochemical analysis of Histone H2B staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of Histone H2B staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Histone H2B Antibody (Acetyl-Lys5) LS-C358766
Rabbit Polyclonal to Human Histone H2B
Chicken, Zebrafish, Mouse, Dog, Bovine, Human, Monkey
ICC, IF, IHC, IHC-P, WB
Unconjugated
30µl 1mg/ml  $215.00; 100µl 1mg/ml  $265.00; 200µl 1mg/ml  $325.00
Immunohistochemical analysis of Histone H2B (AcK5) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of Histone H2B (AcK5) staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

IHC-plus™ Histone H2B Antibody (aa24-73) LS-B12489
Rabbit Polyclonal (IgG) to Human Histone H2B
Mouse, Human
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
100µl 1mg/ml  $505.00
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot analysis of the lysates from HUVECcells using Histone H2B antibody.

Histone H2B Antibody (N-Terminus) LS-C356094
Rabbit Polyclonal to Human Histone H2B
Mouse, Bovine, Rat, Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $245.00
Immunohistochemical analysis of Histone H2B staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of Histone H2B staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Histone H2B Antibody (Ubiquityl-Lys120, clone B1249M) LS-C190903
Rabbit Monoclonal [clone B1249M] (IgG) to Human Histone H2B
Mouse, Rat, Human, Monkey
WB
Unconjugated
20µl  $415.00; 100µl  $695.00

Histone H2B Antibody LS-C416351
Rabbit Polyclonal to Human Histone H2B
Mouse, Human
ICC, IF, IHC, WB
Unconjugated
100µg  $305.00
Western blot of COS7 cell lysate using Histone H2B Antibody

Histone H2B Antibody (Acetyl-Lys12) LS-C120508
Rabbit Polyclonal (IgG) to Human Histone H2B
Mouse, Human
IF, IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Histone H2B (Acetyl-Lys12) Antibody. The picture on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of HeLa cells, using Histone H2B (Acetyl-Lys12) Antibody. The picture on the right is blocked with the synthesized peptide.

Histone H2B Antibody (Acetyl-Lys15) LS-C120509
Rabbit Polyclonal (IgG) to Human Histone H2B
Mouse, Human
IF, IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H2B (Acetyl-Lys15) Antibody. The picture on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of HeLa cells, using Histone H2B (Acetyl-Lys15) Antibody. The picture on the right is blocked with the synthesized peptide.

Histone H2B Antibody (10-90 aa) LS-C380557
Rabbit Polyclonal (IgG) to Human Histone H2B
Mouse, Human, Monkey
ELISA, IF, IHC, WB
Unconjugated
50µl 1mg/ml  $235.00; 100µl 1mg/ml  $265.00; 200µl 1mg/ml  $335.00

Histone H2B Antibody (1-80 aa) LS-C380559
Rabbit Polyclonal (IgG) to Human Histone H2B
Mouse, Human
ELISA, IF, IHC, WB
Unconjugated
50µl 1mg/ml  $235.00; 100µl 1mg/ml  $265.00; 200µl 1mg/ml  $335.00
Western blot of Acetyl-Histone H2B (K15) antibody

Histone H2B Antibody (10-90 aa, N-terminal) LS-C383941
Rabbit Polyclonal (IgG) to Human Histone H2B
Mouse, Human
ELISA, IF, IHC, WB
Unconjugated
50µl 1mg/ml  $235.00; 100µl 1mg/ml  $265.00; 200µl 1mg/ml  $335.00
Western blot of Histone H2B antibody

Histone H2B Antibody (Acetyl-Lys20) LS-C24278
Rabbit Polyclonal (IgG) to Human Histone H2B
Mouse, Rat, Human
ELISA, ICC, IHC, IHC-P, IP, WB
Unconjugated
100µl  $575.00

Histone H2B Antibody (Acetyl-Lys5) LS-C24279
Rabbit Polyclonal (IgG) to Human Histone H2B
Mouse, Rat, Human, Monkey
ELISA, ICC, IHC, IHC-P, IP, WB
Unconjugated
100µl  $575.00
Showing 1-25 of 145 results

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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).