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IHCPlus™ Histone H2B Antibody Acetyl‑Lys12 Polyclonal IHC,WB LS‑B2446

Catalog Number Size Price
LS-B2446-50 50 µl $425 
Anti-Histone H2B antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

IHCPlus™ Histone H2B Antibody Acetyl‑Lys12 Polyclonal IHC,WB LS‑B2446

Note: This antibody replaces LS-C24283
Antibody:
Rabbit Polyclonal to Human Histone H2B
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat, Chicken
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B2446-50
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human Histone H2B
Application:
IHC, IHC-P, WB
Reactivity:
Human, Mouse, Rat, Chicken
Format:
Unconjugated, Unmodified

Specifications

Description
Histone H2B antibody LS-B2446 is an unconjugated rabbit polyclonal antibody to Histone H2B from human, mouse, rat and other species. Validated for IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human Histone H2B
Host
Rabbit
Reactivity
Human, Mouse, Rat, Chicken (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Antiserum
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100)
  • Western blot (1:1000 - 1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
KLH-conjugated, synthetic peptide APAPKAcKGSKKAITK corresponding to 7-20 amino acid of human Histone H2B member F. The immunizing sequence has 13 out of 14 identical amino acids in mouse, chicken and rat.
Epitope
Acetyl-Lys12
Specificity
Recognizes human acetylated Histone 2B, Mr 15kD. Species sequence Homology: mouse, chicken and rat.
Usage
Immunohistochemistry: LS-B2446 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2446 was determined to be 1:100.
Presentation
Serum, 0.05% Sodium Azide, 30% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

Validation

Anti-Histone H2B antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
IHC Validated
Anti-Histone H2B antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-Histone H2B antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

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Requested From: United States
Date Requested: 9/18/2019