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Anti-His Tag Antibody LS-C60077


Wt. Vol. Conc. Price
100 µg - 1 mg/ml $315
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

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100% Guaranteed 100% Guaranteed Publications Publications (1)
Rabbit Polyclonal
Western blot, ELISA


His Tag
Affinity purified


  • Western blot (1:1000 - 1:3000)
  • ELISA (1:10000 - 1:40000)

Specificity and Use

His Tag antibody was raised against 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
the 6X His motif and is useful in determining its presence in various assays. This polyclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag. To date, this antibody has reacted with all His tagged proteins so far tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins. The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells
This antibody has been tested by ELISA and western blotting against both the immunizing peptide and His-containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.


0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Store at -20°C. Aliquot to avoid freeze/thaw cycles. Store undiluted.
For research use only.

Publications (1)

Single amino acid substitutions in the severe acute respiratory syndrome coronavirus spike glycoprotein determine viral entry and immunogenicity of a major neutralizing domain. Yi CE, Ba L, Zhang L, Ho DD, Chen Z. Journal of virology. 2005 79:11638-46. [PubMed:16140741] [PMC:PMC1212612]

About His Tag

A polyhistidine-tag is an amino acid motif in proteins that consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein.

Western blot

Western blot
Anti-6X His Antibody - Western Blot. Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins by western blot. Polyclonal rabbit.

Requested From: United States
Date Requested: 10/28/2016

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