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| Catalog Number | Size | Price |
|---|---|---|
| LS-C57342-0.1 | 0.1 mg (1 mg/ml) | $332 |
Monoclonal Mouse anti‑Synthetic His Tag Antibody (clone AD1.1.10, WB) LS‑C57342
Monoclonal Mouse anti‑Synthetic His Tag Antibody (clone AD1.1.10, WB) LS‑C57342
Note: This antibody replaces LS-C57339, LS-C188122
Antibody:
His Tag Mouse anti-Synthetic Monoclonal (AD1.1.10) Antibody
Application:
WB, IP, ELISA
Reactivity:
Synthetic
Format:
Unconjugated, Unmodified
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
His Tag Mouse anti-Synthetic Monoclonal (AD1.1.10) Antibody
Application:
WB, IP, ELISA
Reactivity:
Synthetic
Format:
Unconjugated, Unmodified
Specifications
Description
His Tag antibody LS-C57342 is an unconjugated mouse monoclonal antibody to synthetic His Tag. Validated for ELISA, IP and WB.
Host
Mouse
Reactivity
Synthetic
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
Clone
AD1.1.10
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
PAX6 transcription factor linked to histidine tag.
Specificity
Recognizes proteins and peptides containing the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. Clone AD1.1.10 has been used to detect and purify histidine-tagged proteins expressed in mammalian and non-mammalian ; ; and cell lines. In Western blotting of bacterial extracts the antibody has been shown not to cross-react with any endogenous products, although some cross-reactivity may be seen with extracts of insect or mammalian cells. Is routinely tested in Western blotting on histidine tagged recombinant proteins and reacts against all histidine-tagged proteins so far tested.
Applications
- Western blot
- Immunoprecipitation
- ELISA
Presentation
PBS, 0.09% Sodium Azide
Storage
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Restrictions
For research use only. Intended for use by laboratory professionals.
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Synthetic
Applications:
Immunofluorescence, Flow Cytometry
![His Tag Antibody - Anti-6X His epitope tag Antibody - Western Blot. Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins by western blot. Polyclonal Rabbit-anti-6X His epitope tag at 0.5-1.0 ug/ml was used to detect 1.0 ug of recombinant protein containing the His epitope tag. A 4-20% gradient gel was used to resolve the protein by SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking, the membrane was probed with the primary antibody for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG [H&L] MX10 (code for 30 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results. This image was taken for the unconjugated form of this product. Other forms have not been tested.](https://lsbio-7d62.kxcdn.com//image2/his-tag-antibody-hrp-ls-c154274/81964_281469.jpg)
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 5/10/2024
Date Requested: 5/10/2024
