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Anti-HIF1A / HIF1 Alpha Antibody (aa530-825) IHC-plus™ LS-B495

Note: This antibody replaces LS-C2558


Wt. Vol. Conc. Price
- 100 µl 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Alternate Forms of this Antibody

LS-C83732 HRP Conjugated
LS-C141170 DY650 Conjugated
LS-C141171 DY488 Conjugated
LS-C141172 DY550 Conjugated
LS-C360376 AF647 Conjugated
LS-C360379 FITC Conjugated
LS-C360380 AF405 Conjugated
LS-C360382 AF700 Conjugated
LS-C360385 DY405 Conjugated
LS-C360426 AF488 Conjugated

100% Guaranteed 100% Guaranteed Publications Publications (73)
Rabbit Polyclonal to Mouse HIF1A / HIF1 Alpha
Mouse, Human, Rat, Primate
IHC - Paraffin, Western blot


Mouse HIF1A / HIF1 Alpha
Mouse, Human, Rat, Primate (tested or 100% immunogen sequence identity)
Immunoaffinity purified


IHC - Paraffin (5 - 10 µg/ml), Western blot (1:500 - 1:2000)

Specificity and Use

HIF1A / HIF1 Alpha antibody was raised against a fusion protein including residues 530-825 of the mouse HIF-1 alpha protein
HIF-1 alpha.
Immunohistochemistry: LS-B495 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B495 was determined to be 5-10 ug/ml.


PBS, 0.05% sodium azide
Store at +4°C. DO NOT FREEZE.
For research use only.

Publications (73)

[Interrelationships between methylmercury exposure and selenium supplementation in cockerels using selected laboratory diagnostic parameters and toxicological parameters of residues]. Wilsdorf G, Schulz O, Walzel R. DTW. Deutsche tierrztliche Wochenschrift. 1991 98:373-6. (WB; Mouse) [PubMed:1752206]
The sail sign. Chapman S. British journal of hospital medicine. 1991 46:399-400. (WB; Mouse) [PubMed:1760676]
Increased expression of HIF-1alpha, nNOS, and VEGF in the cerebral cortex of anemic rats. McLaren AT, Marsden PA, Mazer CD, Baker AJ, Stewart DJ, Tsui AK, Li X, Yucel Y, Robb M, Boyd SR, Liu E, Yu J, Hare GM. American journal of physiology. Regulatory, integrative and comparative physiology. 2007 292:R403-14. (ICC, IHC, WB, IHC-P; Rat) [PubMed:16973934]
Synergistic up-regulation of vascular endothelial growth factor (VEGF) expression in macrophages by adenosine A2A receptor agonists and endotoxin involves transcriptional regulation via the hypoxia response element in the VEGF promoter. Ramanathan M, Pinhal-Enfield G, Hao I, Leibovich SJ. Molecular biology of the cell. 2007 18:14-23. (WB; Mouse) [PubMed:17065555] [PMC:PMC1751314]
Hypoxia-inducible factor-1alpha is a key regulator of metastasis in a transgenic model of cancer initiation and progression. Liao D, Corle C, Seagroves TN, Johnson RS. Cancer research. 2007 67:563-72. (WB; Mouse, Human) [PubMed:17234764]
Somatic inactivation of the PHD2 prolyl hydroxylase causes polycythemia and congestive heart failure. Minamishima YA, Moslehi J, Bardeesy N, Cullen D, Bronson RT, Kaelin WG. Blood. 2008 111:3236-44. (WB; Mouse) [PubMed:18096761] [PMC:PMC2265460]
High oxygen prevents fetal lethality due to lack of catecholamines. Ream MA, Chandra R, Peavey M, Ray AM, Roffler-Tarlov S, Kim HG, Wetsel WC, Rockman HA, Chikaraishi DM. American journal of physiology. Regulatory, integrative and comparative physiology. 2008 295:R942-53. (WB; Mouse) [PubMed:18635452] [PMC:PMC2536863]
HIF1alpha is essential for normal intrauterine differentiation of alveolar epithelium and surfactant production in the newborn lung of mice. Saini Y, Harkema JR, LaPres JJ. The Journal of biological chemistry. 2008 283:33650-7. (WB; Mouse) [PubMed:18801745] [PMC:PMC2586263]
Impaired DNA double-strand break repair contributes to chemoresistance in HIF-1 alpha-deficient mouse embryonic fibroblasts. Wirthner R, Wrann S, Balamurugan K, Wenger RH, Stiehl DP. Carcinogenesis. 2008 29:2306-16. (WB; Mouse) [PubMed:18842680]
An anti-vascular endothelial growth factor receptor 2/fetal liver kinase-1 Listeria monocytogenes anti-angiogenesis cancer vaccine for the treatment of primary and metastatic Her-2/neu+ breast tumors in a mouse model. Seavey MM, Maciag PC, Al-Rawi N, Sewell D, Paterson Y. Journal of immunology (Baltimore, Md. : 1950). 2009 182:5537-46. (ICC, IHC; Mouse) [PubMed:19380802] [PMC:PMC2850569]
Iron transporters are differentially regulated by dietary iron, and modifications are associated with changes in manganese metabolism in young pigs. Hansen SL, Trakooljul N, Liu HC, Moeser AJ, Spears JW. The Journal of nutrition. 2009 139:1474-9. [PubMed:19535423]
Obesity and metabolic syndrome in histone demethylase JHDM2a-deficient mice. Inagaki T, Tachibana M, Magoori K, Kudo H, Tanaka T, Okamura M, Naito M, Kodama T, Shinkai Y, Sakai J. Genes to cells : devoted to molecular & cellular mechanisms. 2009 14:991-1001. (WB; Mouse) [PubMed:19624751]
A feedback loop involving the Phd3 prolyl hydroxylase tunes the mammalian hypoxic response in vivo. Minamishima YA, Moslehi J, Padera RF, Bronson RT, Liao R, Kaelin WG. Molecular and cellular biology. 2009 29:5729-41. (WB; Mouse) [PubMed:19720742] [PMC:PMC2772748]
HIF-1alpha and HIF-2alpha are differentially regulated in vivo in neuroblastoma: high HIF-1alpha correlates negatively to advanced clinical stage and tumor vascularization. Noguera R, Fredlund E, Piqueras M, Pietras A, Beckman S, Navarro S, Phlman S. Clinical cancer research : an official journal of the American Association for Cancer Research. 2009 15:7130-6. [PubMed:19903792]
Role of hypoxia-inducible factor 1{alpha} in modulating cobalt-induced lung inflammation. Saini Y, Kim KY, Lewandowski R, Bramble LA, Harkema JR, Lapres JJ. American journal of physiology. Lung cellular and molecular physiology. 2010 298:L139-47. [PubMed:19915160] [PMC:PMC2822559]
Hypoxia-regulated delta-like 1 homologue enhances cancer cell stemness and tumorigenicity. Kim Y, Lin Q, Zelterman D, Yun Z. Cancer research. 2009 69:9271-80. [PubMed:19934310] [PMC:PMC2828615]
Hearts of hypoxia-inducible factor prolyl 4-hydroxylase-2 hypomorphic mice show protection against acute ischemia-reperfusion injury. Hyvrinen J, Hassinen IE, Sormunen R, Mki JM, Kivirikko KI, Koivunen P, Myllyharju J. The Journal of biological chemistry. 2010 285:13646-57. [PubMed:20185832] [PMC:PMC2859527]
Retina-specific activation of a sustained hypoxia-like response leads to severe retinal degeneration and loss of vision. Lange C, Caprara C, Tanimoto N, Beck S, Huber G, Samardzija M, Seeliger M, Grimm C. Neurobiology of disease. 2011 41:119-30. [PubMed:20817091]
Tissue inhibitor of metalloproteinases-1-induced scattered liver metastasis is mediated by hypoxia-inducible factor-1. Schelter F, Halbgewachs B, Bumler P, Neu C, Grlach A, Schrtzlmair F, Krger A. Clinical & experimental metastasis. 2011 28:91-9. (WB, IHC-Fr; Mouse) [PubMed:21053058]
HIF1A is essential for the development of the intermediate plexus of the retinal vasculature. Caprara C, Thiersch M, Lange C, Joly S, Samardzija M, Grimm C. Investigative ophthalmology & visual science. 2011 52:2109-17. [PubMed:21212189]
Cardiomyocyte-specific prolyl-4-hydroxylase domain 2 knock out protects from acute myocardial ischemic injury. Hlscher M, Silter M, Krull S, von Ahlen M, Hesse A, Schwartz P, Wielockx B, Breier G, Katschinski DM, Zieseniss A. The Journal of biological chemistry. 2011 286:11185-94. [PubMed:21270129] [PMC:PMC3064173]
Ischaemia-induced retinal neovascularisation and diabetic retinopathy in mice with conditional knockout of hypoxia-inducible factor-1 in retinal Mller cells. Lin M, Chen Y, Jin J, Hu Y, Zhou KK, Zhu M, Le YZ, Ge J, Johnson RS, Ma JX. Diabetologia. 2011 54:1554-66. [PubMed:21360191]
Normoxic activation of hypoxia-inducible factors in photoreceptors provides transient protection against light-induced retinal degeneration. Lange C, Heynen SR, Tanimoto N, Thiersch M, Le YZ, Meneau I, Seeliger MW, Samardzija M, Caprara C, Grimm C. Investigative ophthalmology & visual science. 2011 52:5872-80. [PubMed:21447692]
Hypoxia-inducible factor-1 signalling promotes goblet cell hyperplasia in airway epithelium. Polosukhin VV, Cates JM, Lawson WE, Milstone AP, Matafonov AG, Massion PP, Lee JW, Randell SH, Blackwell TS. The Journal of pathology. 2011 224:203-11. [PubMed:21557221]
Pre-treatment with isoflurane ameliorates renal ischemic-reperfusion injury in mice. Zhang L, Huang H, Cheng J, Liu J, Zhao H, Vizcaychipi MP, Ma D. Life sciences. 2011 88:1102-7. (WB; Mouse) [PubMed:21565202]
Differential hypoxic regulation of hypoxia-inducible factors 1alpha and 2alpha. Lin Q, Cong X, Yun Z. Molecular cancer research : MCR. 2011 9:757-65. (WB; Human) [PubMed:21571835] [PMC:PMC3117969]
The role of hypoxia-inducible factor-1 in acetaminophen hepatotoxicity. Sparkenbaugh EM, Saini Y, Greenwood KK, LaPres JJ, Luyendyk JP, Copple BL, Maddox JF, Ganey PE, Roth RA. The Journal of pharmacology and experimental therapeutics. 2011 338:492-502. [PubMed:21576378] [PMC:PMC3141908]
Scaffold porosity and oxygenation of printed hydrogel constructs affect functionality of embedded osteogenic progenitors. Fedorovich NE, Kuipers E, Gawlitta D, Dhert WJ, Alblas J. Tissue engineering. Part A. 2011 17:2473-86. (IHC; Goat) [PubMed:21599540]
IGF binding protein-6 expression in vascular endothelial cells is induced by hypoxia and plays a negative role in tumor angiogenesis. Zhang C, Lu L, Li Y, Wang X, Zhou J, Liu Y, Fu P, Gallicchio MA, Bach LA, Duan C. International journal of cancer. Journal international du cancer. 2012 130:2003-12. (WB; Human) [PubMed:21618524] [PMC:PMC3259243]
MCM proteins are negative regulators of hypoxia-inducible factor 1. Hubbi ME, Luo W, Baek JH, Semenza GL. Molecular cell. 2011 42:700-12. (WB, IP; Human) [PubMed:21658608] [PMC:PMC3131976]
Two mutations impair the stability and function of ubiquitin-activating enzyme (E1). Lao T, Chen S, Sang N. Journal of cellular physiology. 2012 227:1561-8. [PubMed:21678405] [PMC:PMC3184329]
Dimethyloxalylglycine stabilizes HIF-1 in cultured human endothelial cells and increases random-pattern skin flap survival in vivo. Shafighi M, Olariu R, Fathi AR, Djafarzadeh S, Jakob SM, Banic A, Constantinescu MA. Plastic and reconstructive surgery. 2011 128:415-22. (WB; Human) [PubMed:21788833]
The Loss of HIF1 Leads to Increased Susceptibility to Cadmium-Chloride-Induced Toxicity in Mouse Embryonic Fibroblasts. Vengellur A, Grier E, Lapres JJ. Journal of toxicology. 2011 2011:391074. [PubMed:21811500] [PMC:PMC3147003]
Activation of hypoxia-inducible factor-1 protects airway epithelium against oxidant-induced barrier dysfunction. Olson N, Hristova M, Heintz NH, Lounsbury KM, van der Vliet A. American journal of physiology. Lung cellular and molecular physiology. 2011 301:L993-L1002. [PubMed:21926263] [PMC:PMC3233830]
Cell-autonomous and non-cell-autonomous neuroprotective functions of ROR in neurons and astrocytes during hypoxia. Jolly S, Journiac N, Naudet F, Gautheron V, Mariani J, Vernet-der Garabedian B. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2011 31:14314-23. [PubMed:21976517]
Genetic and functional studies implicate HIF1 as a 14q kidney cancer suppressor gene. Shen C, Beroukhim R, Schumacher SE, Zhou J, Chang M, Signoretti S, Kaelin WG. Cancer discovery. 2011 1:222-35. [PubMed:22037472] [PMC:PMC3202343]
Inhibition of prolyl hydroxylases by dimethyloxaloylglycine after stroke reduces ischemic brain injury and requires hypoxia inducible factor-1. Ogle ME, Gu X, Espinera AR, Wei L. Neurobiology of disease. 2012 45:733-42. [PubMed:22061780] [PMC:PMC3286647]
Identification of valid housekeeping genes for quantitative RT-PCR analysis of cardiosphere-derived cells preconditioned under hypoxia or with prolyl-4-hydroxylase inhibitors. Tan SC, Carr CA, Yeoh KK, Schofield CJ, Davies KE, Clarke K. Molecular biology reports. 2012 39:4857-67. (WB; Rat) [PubMed:22065248] [PMC:PMC3294216]
GSK-3 regulates cell growth, migration, and angiogenesis via Fbw7 and USP28-dependent degradation of HIF-1. Flgel D, Grlach A, Kietzmann T. Blood. 2012 119:1292-301. [PubMed:22144179] [PMC:PMC3352078]
Hypoxia-inducible factor 1 promotes primary tumor growth and tumor-initiating cell activity in breast cancer. Schwab LP, Peacock DL, Majumdar D, Ingels JF, Jensen LC, Smith KD, Cushing RC, Seagroves TN. Breast cancer research : BCR. 2012 14:R6. (WB; Mouse) [PubMed:22225988] [PMC:PMC3496121]
Transient ureteral obstruction prevents against kidney ischemia/reperfusion injury via hypoxia-inducible factor (HIF)-2 activation. Zhang S, Han CH, Chen XS, Zhang M, Xu LM, Zhang JJ, Xia Q. PloS one. 2012 7:e29876. [PubMed:22295069] [PMC:PMC3266244]
A new pharmacological agent (AKB-4924) stabilizes hypoxia inducible factor-1 (HIF-1) and increases skin innate defenses against bacterial infection. Okumura CY, Hollands A, Tran DN, Olson J, Dahesh S, von Kckritz-Blickwede M, Thienphrapa W, Corle C, Jeung SN, Kotsakis A, Shalwitz RA, Johnson RS, Nizet V. Journal of molecular medicine (Berlin, Germany). 2012 90:1079-89. (WB; Mouse) [PubMed:22371073] [PMC:PMC3606899]
HIF-1 and c-Src mediate increased glucose uptake induced by endothelin-1 and connexin43 in astrocytes. Valle-Casuso JC, Gonzlez-Snchez A, Medina JM, Tabernero A. PloS one. 2012 7:e32448. [PubMed:22384254] [PMC:PMC3285680]
Hypoxia promotes dissemination of multiple myeloma through acquisition of epithelial to mesenchymal transition-like features. Azab AK, Hu J, Quang P, Azab F, Pitsillides C, Awwad R, Thompson B, Maiso P, Sun JD, Hart CP, Roccaro AM, Sacco A, Ngo HT, Lin CP, Kung AL, Carrasco RD, Vanderkerken K, Ghobrial IM. Blood. 2012 119:5782-94. [PubMed:22394600] [PMC:PMC3382938]
Systemic elevation of PTEN induces a tumor-suppressive metabolic state. Garcia-Cao I, Song MS, Hobbs RM, Laurent G, Giorgi C, de Boer VC, Anastasiou D, Ito K, Sasaki AT, Rameh L, Carracedo A, Vander Heiden MG, Cantley LC, Pinton P, Haigis MC, Pandolfi PP. Cell. 2012 149:49-62. (WB; Mouse) [PubMed:22401813] [PMC:PMC3319228]
Adrenomedullin promotes human endothelial cell proliferation via HIF-1. Chen L, Qiu JH, Zhang LL, Luo XD. Molecular and cellular biochemistry. 2012 365:263-73. (IHC, WB; Human) [PubMed:22406979]
Blockade of p53 by HIF-2, but not HIF-1, is involved in arsenite-induced malignant transformation of human bronchial epithelial cells. Xu Y, Li Y, Pang Y, Ling M, Shen L, Jiang R, Zhao Y, Zhou J, Wang X, Liu Q. Archives of toxicology. 2012 86:947-59. (WB; Human) [PubMed:22447124]
Testosterone replacement therapy promotes angiogenesis after acute myocardial infarction by enhancing expression of cytokines HIF-1a, SDF-1a and VEGF. Chen Y, Fu L, Han Y, Teng Y, Sun J, Xie R, Cao J. European journal of pharmacology. 2012 684:116-24. (IHC; Rat) [PubMed:22484505]
UBXN7 docks on neddylated cullin complexes using its UIM motif and causes HIF1 accumulation. Bandau S, Knebel A, Gage ZO, Wood NT, Alexandru G. BMC biology. 2012 10:36. (WB; Human) [PubMed:22537386] [PMC:PMC3349548]
Intranasal deferoxamine improves performance in radial arm water maze, stabilizes HIF-1, and phosphorylates GSK3 in P301L tau transgenic mice. Fine JM, Baillargeon AM, Renner DB, Hoerster NS, Tokarev J, Colton S, Pelleg A, Andrews A, Sparley KA, Krogh KM, Frey WH, Hanson LR. Experimental brain research. Experimentelle Hirnforschung. Exprimentation crbrale. 2012 219:381-90. (WB; Mouse) [PubMed:22547371]
Ischemia is the prime but not the only cause of human multipotent stromal cell death in tissue-engineered constructs in vivo. Becquart P, Cambon-Binder A, Monfoulet LE, Bourguignon M, Vandamme K, Bensidhoum M, Petite H, Logeart-Avramoglou D. Tissue engineering. Part A. 2012 18:2084-94. (IHC; Human) [PubMed:22578283]
Von Hippel-Lindau protein in the RPE is essential for normal ocular growth and vascular development. Lange CA, Luhmann UF, Mowat FM, Georgiadis A, West EL, Abrahams S, Sayed H, Powner MB, Fruttiger M, Smith AJ, Sowden JC, Maxwell PH, Ali RR, Bainbridge JW. Development (Cambridge, England). 2012 139:2340-50. (ICC, IHC; Mouse) [PubMed:22627278] [PMC:PMC3367444]
Cells lacking the fumarase tumor suppressor are protected from apoptosis through a hypoxia-inducible factor-independent, AMPK-dependent mechanism. Bardella C, Olivero M, Lorenzato A, Geuna M, Adam J, O'Flaherty L, Rustin P, Tomlinson I, Pollard PJ, Di Renzo MF. Molecular and cellular biology. 2012 32:3081-94. (WB; Human) [PubMed:22645311] [PMC:PMC3434507]
Acute hypoxia induces apoptosis of pancreatic -cell by activation of the unfolded protein response and upregulation of CHOP. Zheng X, Zheng X, Wang X, Ma Z, Gupta Sunkari V, Botusan I, Takeda T, Bjrklund A, Inoue M, Catrina SB, Brismar K, Poellinger L, Pereira TS. Cell death & disease. 2012 3:e322. (WB; Mouse) [PubMed:22695615] [PMC:PMC3388238]
Involvement of decreased hypoxia-inducible factor 1 activity and resultant G(1)-S cell cycle transition in radioresistance of perinecrotic tumor cells. Qi Y, Tian X, Liu J, Han Y, Graham AM, Simon MC, Penninger JM, Carmeliet P, Li S. Oncogene. 2012 (IHC; Human) [PubMed:22710721]
A novel role for the transcription factor HIF-1 in the formation of mast cell extracellular traps. Branitzki-Heinemann K, Okumura CY, Vllger L, Kawakami Y, Kawakami T, Naim HY, Nizet V, Von Kckritz-Blickwede M. The Biochemical journal. 2012 446:159-63. (ICC; Human) [PubMed:22738198] [PMC:PMC3606900]
Bnip3 and AIF cooperate to induce apoptosis and cavitation during epithelial morphogenesis. Qi Y, Tian X, Liu J, Han Y, Graham AM, Simon MC, Penninger JM, Carmeliet P, Li S. The Journal of cell biology. 2012 198:103-14. (WB, IP, ChrIP; Mouse) [PubMed:22753893] [PMC:PMC3392936]
Abnormal vascularization in mouse retina with dysregulated retinal cholesterol homeostasis. Omarova S, Charvet CD, Reem RE, Mast N, Zheng W, Huang S, Peachey NS, Pikuleva IA. The Journal of clinical investigation. 2012 122:3012-23. (ICC, IHC-Fr; Mouse) [PubMed:22820291] [PMC:PMC3408752]
Attenuation of reactive oxygen species by antioxidants suppresses hypoxia-induced epithelial-mesenchymal transition and metastasis of pancreatic cancer cells. Shimojo Y, Akimoto M, Hisanaga T, Tanaka T, Tajima Y, Honma Y, Takenaga K. Clinical & experimental metastasis. 2013 30:143-54. (ICC; Human) [PubMed:22833345]
Exposure to cigarette smoke induces overexpression of von Hippel-Lindau tumor suppressor in mouse skeletal muscle. Basic VT, Tadele E, Elmabsout AA, Yao H, Rahman I, Sirsj A, Abdel-Halim SM. American journal of physiology. Lung cellular and molecular physiology. 2012 303:L519-27. (WB; Mouse) [PubMed:22842216] [PMC:PMC3468481]
Hypoxia-inducible factor-1 (HIF1) switches on transient receptor potential ankyrin repeat 1 (TRPA1) gene expression via a hypoxia response element-like motif to modulate cytokine release. Hatano N, Itoh Y, Suzuki H, Muraki Y, Hayashi H, Onozaki K, Wood IC, Beech DJ, Muraki K. The Journal of biological chemistry. 2012 287:31962-72. (WB, ChrIP; Human) [PubMed:22843691] [PMC:PMC3442528]
Downregulation of miR-205 modulates cell susceptibility to oxidative and endoplasmic reticulum stresses in renal tubular cells. Muratsu-Ikeda S, Nangaku M, Ikeda Y, Tanaka T, Wada T, Inagi R. PloS one. 2012 7:e41462. (WB; Human) [PubMed:22859986] [PMC:PMC3408438]
Anthracycline inhibits recruitment of hypoxia-inducible transcription factors and suppresses tumor cell migration and cardiac angiogenic response in the host. Tanaka T, Yamaguchi J, Shoji K, Nangaku M. The Journal of biological chemistry. 2012 287:34866-82. (WB, IP; Human) [PubMed:22908232] [PMC:PMC3471702]
Hypoxia-inducible factor-1 (HIF-1) but not HIF-2 is essential for hypoxic induction of collagen prolyl 4-hydroxylases in primary newborn mouse epiphyseal growth plate chondrocytes. Aro E, Khatri R, Gerard-O'Riley R, Mangiavini L, Myllyharju J, Schipani E. The Journal of biological chemistry. 2012 287:37134-44. (WB; Mouse) [PubMed:22930750] [PMC:PMC3481313]
Aldosterone deficiency adversely affects pregnancy outcome in mice. Todkar A, Di Chiara M, Loffing-Cueni D, Bettoni C, Mohaupt M, Loffing J, Wagner CA. Pflgers Archiv : European journal of physiology. 2012 464:331-43. (WB; Mouse) [PubMed:22941338]
Hypoxia markers are expressed in interneurons exposed to recurrent seizures. Gualtieri F, Marinelli C, Longo D, Pugnaghi M, Nichelli PF, Meletti S, Biagini G. Neuromolecular medicine. 2013 15:133-46. (ICC, IHC; Human) [PubMed:23073716]
The critical role of mast cell-derived hypoxia-inducible factor-1 in human and mice melanoma growth. Jeong HJ, Oh HA, Nam SY, Han NR, Kim YS, Kim JH, Lee SJ, Kim MH, Moon PD, Kim HM, Oh HA. International journal of cancer. Journal international du cancer. 2013 132:2492-501. (IHC, WB; Mouse, Human) [PubMed:23161568]
Sprouty2 expression controls endothelial monolayer integrity and quiescence. Peier M, Walpen T, Christofori G, Battegay E, Humar R. Angiogenesis. 2013 16:455-68. (WB; Mouse) [PubMed:23232625]
Endoplasmic reticulum stress signal impairs erythropoietin production: a role for ATF4. Chiang CK, Nangaku M, Tanaka T, Iwawaki T, Inagi R. American journal of physiology. Cell physiology. 2013 304:C342-53. (WB, ChrIP; Human) [PubMed:23242184]
miR-214 and hypoxia down-regulate Necl-2/CADM1 and enhance ErbB2/ErbB3 signaling. Momose K, Minami A, Shimono Y, Mizutani K, Nobutani K, Azuma T, Takai Y. Genes to cells : devoted to molecular & cellular mechanisms. 2013 18:195-202. (WB; Human) [PubMed:23301758]
Carbon ion radiotherapy of human lung cancer attenuates HIF-1 signaling and acts with considerably enhanced therapeutic efficiency. Subtil FS, Wilhelm J, Bill V, Westholt N, Rudolph S, Fischer J, Scheel S, Seay U, Fournier C, Taucher-Scholz G, Scholz M, Seeger W, Engenhart-Cabillic R, Rose F, Dahm-Daphi J, H[Character e4]nze J. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2014 Mar;28:1412-21. [Full Text Article] [PubMed:24347608]
HIF-1 alpha signaling is augmented during intermittent hypoxia by induction of the Nrf2 pathway in NOX1-expressing adenocarcinoma A549 cells. Malec V, Gottschald OR, Li S, Rose F, Seeger W, H[Character e4]nze J. Free radical biology & medicine. 2010 Jun;48:1626-35. [Full Text Article] [PubMed:20347035]
Loss of HIF-1 alpha in the notochord results in cell death and complete disappearance of the nucleus pulposus. Merceron C, Mangiavini L, Robling A, Wilson TL, Giaccia AJ, Shapiro IM, Schipani E, Risbud MV. PloS one. 9:e110768. [Full Text Article] [PubMed:25338007] [PMC:PMC4206488]

About HIF1A / HIF1 Alpha

Q16665 NM_001530 NP_001521.1

HIF1A Antibody, ARNT-interacting protein Antibody, ARNT interacting protein Antibody, BHLHe78 Antibody, HIF1 Alpha Antibody, Hypoxia-inducible factor1alpha Antibody, HIF-1A Antibody, HIF1 Antibody, MOP1 Antibody, Member of PAS superfamily 1 Antibody, PASD8 Antibody, HIF-1-alpha Antibody, HIF-1alpha Antibody, HIF1-ALPHA Antibody, Member of PAS protein 1 Antibody

Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease.


Anti-HIF1A antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B495 concentration 10 ug/ml.

Requested From: United States
Date Requested: 2/12/2016

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