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Anti-FTO Antibody (N-Terminus) IHC-plus™ LS-B5891

Note: This antibody replaces LS-C134886
Catalog Size Price
LS-B5891-50 50 µg (1 mg/ml) Unavailable

Related Products

36 FTO Antibodies

Most Popular FTO Antibodies

Anti-FTO Antibody (N-Terminus) IHC-plus™ LS-B5891
Rabbit Polyclonal to Mouse FTO
Mouse, Human, Monkey, Rat, Bovine, Hamster, Horse, Pig, Sheep
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-FTO Antibody (aa400-505) IHC-plus™ LS-B7788
Rabbit Polyclonal to Human FTO
Human, Mouse, Primate
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-FTO Antibody (clone 5-2H10) IHC-plus™ LS-B7982
Mouse Monoclonal [clone 5-2H10] (IgG) to Rat FTO
Rat, Human, Mouse, Bovine, Dog, Horse, Sheep, Primate
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image
Anti-FTO Antibody (clone AG103) LS-C344739
Mouse Monoclonal [clone AG103] (IgG2a,k) to Human FTO
Human
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image
Anti-FTO Antibody (clone FT62-6) LS-C344911
Mouse Monoclonal [clone FT62-6] (IgG1,k) to Mouse FTO
Mouse
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed
Rabbit Polyclonal to Mouse FTO
Mouse, Human, Monkey, Rat, Bovine, Hamster, Horse, Pig, Sheep
IHC - Paraffin, Western blot (applications tested for the base form of this product only)
Unconjugated

Details

Mouse FTO
Rabbit
Mouse, Human, Monkey, Rat, Bovine, Hamster, Horse, Pig, Sheep (tested or 100% immunogen sequence identity)
Rabbit (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (0.2 - 1 µg/ml)
  • (applications tested for the base form of this product only)

Specificity and Use

FTO antibody was raised against synthetic peptide from N-Terminus of mouse Fto (Q8BGW1, NP_036066). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Marmoset, Mouse, Rat, Sheep, Hamster, Panda, Bovine, Horse, Pig (100%); Elephant, Rabbit (92%).
LS-E17206 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B5891. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
N-Terminus
Mouse FTO
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Packaging

Lyophilized from PBS with 2% sucrose
50 µl Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About FTO

Q9C0B1 Q9C0B1

FTO Antibody, KIAA1752 Antibody, Protein fto Antibody

Dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single-stranded RNA containing 3-methyluracil, followed by single-stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Specifically demethylates N(6)-methyladenosine (m6A) RNA, the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes.

Immunohistochemistry

Immunohistochemistry
Anti-FTO antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5891 concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

Western blot
FTO antibody LS-B5891 Western blot of Mouse Brain lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From: 
Date Requested: 12/12/2017

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