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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C344911-50 50 µg $295 
LS-C344911-100 100 µg (1 mg/ml) $345 
FTO Antibody - Immunohistochemical staining of mouse FTO using anti-FTO (mouse), mAb (FT62-6) in mouse tissue (1:500 dilution, 200X). A. Immunoperoxidase staining of formalin-Fixed, paraffin-embedded mouse brain. A-1. Isotype control, mouse brain. B. Immunoperoxidase staining of formalin-Fixed, paraffin-embedded mouse uterus. B-1. Isotype control, mouse uterus.
FTO Antibody - Western blot analysis using anti-FTO (mouse), mAb (FT62-6) at 1:2000 dilution. A). 1: Mouse kidney (200 ug). 2: Mouse spleen (200 ug). 3: Mouse heart (200 ug). 4: Mouse liver (500 ug). 5: Mouse stomach (200 ug). 6: Mouse brain (300 ug). 7: Mouse lung (200 ug). B). 1: Mouse FTO (10ng). 2: 3T3L1 (100 ug).
FTO Antibody - Immunoprecipitation confirmed by recombinant mouse FTO. Recombinant mouse FTO proteins at different concentrations were precipitated using anti-FTO (mouse), mAb (FT62-6) . The precipitated proteins were separated by SDS-PAGE, electroblotted, and visualized by western blot with rabbit anti-mouse FTO pAb. 1. mFTO (His-tagged) recombinant control 50ng (AG-40A-127). 2. mFTO (His-tagged) 10 ug. 3. mFTO (His-tagged) 5 ug. 4. mFTO (His-tagged) 2.5 ug. 5. mFTO (His-tagged) 1.25 ug. 6. mFTO (His-tagged) 0.312 ug. 7. mFTO (His-tagged) 0.156 ug.
FTO Antibody - Immunohistochemical staining of mouse FTO using anti-FTO (mouse), mAb (FT62-6) in mouse tissue (1:500 dilution, 200X). A. Immunoperoxidase staining of formalin-Fixed, paraffin-embedded mouse brain. A-1. Isotype control, mouse brain. B. Immunoperoxidase staining of formalin-Fixed, paraffin-embedded mouse uterus. B-1. Isotype control, mouse uterus.
FTO Antibody - Western blot analysis using anti-FTO (mouse), mAb (FT62-6) at 1:2000 dilution. A). 1: Mouse kidney (200 ug). 2: Mouse spleen (200 ug). 3: Mouse heart (200 ug). 4: Mouse liver (500 ug). 5: Mouse stomach (200 ug). 6: Mouse brain (300 ug). 7: Mouse lung (200 ug). B). 1: Mouse FTO (10ng). 2: 3T3L1 (100 ug).
FTO Antibody - Immunoprecipitation confirmed by recombinant mouse FTO. Recombinant mouse FTO proteins at different concentrations were precipitated using anti-FTO (mouse), mAb (FT62-6) . The precipitated proteins were separated by SDS-PAGE, electroblotted, and visualized by western blot with rabbit anti-mouse FTO pAb. 1. mFTO (His-tagged) recombinant control 50ng (AG-40A-127). 2. mFTO (His-tagged) 10 ug. 3. mFTO (His-tagged) 5 ug. 4. mFTO (His-tagged) 2.5 ug. 5. mFTO (His-tagged) 1.25 ug. 6. mFTO (His-tagged) 0.312 ug. 7. mFTO (His-tagged) 0.156 ug.
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FTO Antibody (clone FT62‑6) LS‑C344911

FTO Antibody (clone FT62‑6) LS‑C344911

Antibody:
Mouse Monoclonal to Mouse FTO
Application:
IHC, IHC-P, WB, IP, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$295 
LS-C344911-50
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Mouse FTO
Application:
IHC, IHC-P, WB, IP, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
FTO antibody LS-C344911 is an unconjugated mouse monoclonal antibody to mouse FTO. Validated for ELISA, IHC, IP and WB.
Target
Mouse FTO
Host
Mouse
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
FT62-6
Conjugations
Unconjugated
Purification
Protein G purified from tissue culture supernatant
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:500)
  • Western blot (1:2000 - 1:5000)
  • Immunoprecipitation (5 µg/ml)
  • ELISA (1:2000 - 1:5000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Recombinant human FTO.
Specificity
Recognizes mouse FTO. Weakly cross-reacts with human FTO. Does not cross-react with rat FTO.
Usage
ELISA - Direct and indirect: 1:2000-1:5000. Immunohistochemistry - Paraffin sections (1:100-1:500. Immunoprecipitation - 5 ug/ml per sample in a final reaction volume of 500-1000ul. Western Blot - 1:2000-1:5000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature.
Presentation
PBS, pH 7.4, no preservatives.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About FTO
Q9C0B1 Q9C0B1

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Requested From: United States
Date Requested: 12/6/2019