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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-C344739-50 50 µg $295 
LS-C344739-100 100 µg $345 
FTO Antibody - Immunohistochemical staining of FTO with anti-FTO (human), mAb (AG103) in brain, hypothalamus and paraventricular nucleus (5~10 ug/ml). This antibody has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin fixed, paraffin-embedded human tissues. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain (40x).
FTO Antibody - Immunoprecipitation of recombinant human FTO proteins using anti-FTO (human), mAb (AG103) at different concentrations. The precipitated proteins were separated by SDS-PAGE, electroblotted, and visualized by Western blot with rabbit anti-mouse FTO pAb . 1. hFTO (His-tagged) recombinant control 100ng (AG-40A-112). 2. hFTO (His-tagged) 5 ug. 3. hFTO (His-tagged) 2.5 ug. 4. hFTO (His-tagged) 1.25 ug. 5. hFTO (His-tagged) 0.625 ug. 6. hFTO (His-tagged) 0.312 ug. 7. hFTO (His-tagged) 0.156 ug. 8. hFTO (His-tagged) 0.078 ug
FTO Antibody - Western blot analysis of human FTO using anti-human FTO mAb (AG103) at 1:1,000 dilution:. 1. hFTO (His-tagged) 100 ng. 2. HEK293T 100 ug. 3. HEK293E 100 ug. 4. HepG2 100 ug. 5. Hep3B 50 ug. 6. VIT1 80 ug. 7. THP1 74 ug. 8. Molt4 96 ug. 9. A549 48 ug
FTO Antibody - Immunohistochemical staining of FTO with anti-FTO (human), mAb (AG103) in brain, hypothalamus and paraventricular nucleus (5~10 ug/ml). This antibody has been tested in immunohistochemistry, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin fixed, paraffin-embedded human tissues. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain (40x).
FTO Antibody - Immunoprecipitation of recombinant human FTO proteins using anti-FTO (human), mAb (AG103) at different concentrations. The precipitated proteins were separated by SDS-PAGE, electroblotted, and visualized by Western blot with rabbit anti-mouse FTO pAb . 1. hFTO (His-tagged) recombinant control 100ng (AG-40A-112). 2. hFTO (His-tagged) 5 ug. 3. hFTO (His-tagged) 2.5 ug. 4. hFTO (His-tagged) 1.25 ug. 5. hFTO (His-tagged) 0.625 ug. 6. hFTO (His-tagged) 0.312 ug. 7. hFTO (His-tagged) 0.156 ug. 8. hFTO (His-tagged) 0.078 ug
FTO Antibody - Western blot analysis of human FTO using anti-human FTO mAb (AG103) at 1:1,000 dilution:. 1. hFTO (His-tagged) 100 ng. 2. HEK293T 100 ug. 3. HEK293E 100 ug. 4. HepG2 100 ug. 5. Hep3B 50 ug. 6. VIT1 80 ug. 7. THP1 74 ug. 8. Molt4 96 ug. 9. A549 48 ug
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FTO Antibody (clone AG103) LS‑C344739

FTO Antibody (clone AG103) LS‑C344739

Antibody:
Mouse Monoclonal to Human FTO
Application:
IHC, IHC-P, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$295 
LS-C344739-50
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human FTO
Application:
IHC, IHC-P, WB, IP, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
FTO antibody LS-C344739 is an unconjugated mouse monoclonal antibody to human FTO. Validated for ELISA, IHC, IP and WB.
Target
Human FTO
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal
Clone
AG103
Conjugations
Unconjugated
Purification
Protein G purified from tissue culture supernatant
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (5 - 10 µg/ml)
  • Western blot (1:2000 - 1:5000)
  • Immunoprecipitation (5 µg/ml)
  • ELISA (1:2000 - 1:5000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Recombinant human FTO.
Specificity
Recognizes human FTO.
Usage
ELISA - Direct and indirect: 1:2000-1:5000. Immunoprecipitation - 5 ug/ml per sample in a final reaction volume of 500-1000ul. Immunohistochemistry - 5-10 ug/ml: paraffin sections. Western Blot - 1:2000-1:5000 using ECL. Suggested blocking and dilution buffer is PBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature.
Presentation
PBS, pH 7.4, no preservatives.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About FTO
Q9C0B1 Q9C0B1

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Requested From: United States
Date Requested: 12/11/2019