Target
Human FAK / Focal Adhesion Kinase
Synonyms
PTK2 | FADK | Fadk1 | Focal adhesion kinase | FRNK | FADK 1 | Focal adhesion kinase 1 | FAK1 | p125FAK | PPP1R71 | Protein-tyrosine kinase 2 | PTK2 protein tyrosine kinase 2 | FAK | pp125 FA kinase | Pp125FAK
Reactivity
Human, Mouse, Rat, Hamster
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated.
Also available conjugated with
APC,
Biotin,
FITC,
HRP,
PE.
Purification
Protein A purified
Immunogen
PGEX-derived fusion protein containing aa748-1053 of human FAK.
Specificity
Recognizes human p125 FAK ; does not cross-react with Pyk2. Species cross-reactivity: mouse, rat and hamster not avian.
Applications
- ICC (10 µg/ml)
- Western blot (0.5 - 2 µg/ml)
- Immunoprecipitation
Usage
WB: Detects FAK in RIPA lysates from murine 3T3 and previously in human A431 RIPA cell lysate. 3T3 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with Rabbit Anti-FAK (1 ug/ml). Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system. ICC: Shows positive immunostaining for Fak in Ref 52 cells fixed with 3.7% formaldehyde. IP: 4ug immunoprecipitates FAK from 500ug of murine 3T3 RIPA lysate.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About FAK / Focal Adhesion Kinase