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| Catalog Number | Size | Price |
|---|---|---|
| LS-A2953-50 | 50 µg (1 mg/ml) | $460 |
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PathPlus™ Polyclonal Rabbit anti‑Human FAK / Focal Adhesion Kinase Antibody (C‑Terminus, IHC) LS‑A2953
PathPlus™ Polyclonal Rabbit anti‑Human FAK / Focal Adhesion Kinase Antibody (C‑Terminus, IHC) LS‑A2953
Note: This antibody replaces LS-C65884, LS-C42639
Antibody:
FAK / Focal Adhesion Kinase Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P
Reactivity:
Human, Monkey, Mouse
Format:
Unconjugated, Unmodified
Overview
Antibody:
FAK / Focal Adhesion Kinase Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P
Reactivity:
Human, Monkey, Mouse
Format:
Unconjugated, Unmodified
Specifications
Description
protein tyrosine kinase 2 (PTK2) plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis. Required for early embryonic development and placenta development. PTK2 regulates numerous signaling pathways. It promotes activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascade as well as activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling cascade. It promotes localized and transient activation of guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs), and thereby modulates the activity of Rho family GTPases.
References: The UniProt Consortium. Nucleic Acids Res. 47: D506-515 (2019); Nucleic Acids Res. 2016 Jan 4;44(D1):D733-45, PMID:26553804
Target
Human FAK / Focal Adhesion Kinase
Synonyms
PTK2 | FADK | Fadk1 | Focal adhesion kinase | FRNK | FADK 1 | Focal adhesion kinase 1 | FAK1 | p125FAK | PPP1R71 | Protein-tyrosine kinase 2 | PTK2 protein tyrosine kinase 2 | FAK | pp125 FA kinase | Pp125FAK
Host
Rabbit
Reactivity
Human, Monkey, Mouse
(tested or 100% immunogen sequence identity)
Predicted
Rat, Bat, Bovine, Dog, Horse, Rabbit (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic 16 amino acid peptide from C-Terminus of human FAK. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse (100%); Rat, Dog, Bat, Bovine, Elephant, Horse, Rabbit, Opossum, Platypus (94%); Hamster, Xenopus (88%); Turkey, Chicken, Pufferfish (81%).
Epitope
C-Terminus
Specificity
Human FAK. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Applications
- IHC
- IHC - Paraffin (6 µg/ml)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 6 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About FAK / Focal Adhesion Kinase
Validation
Lung, non small cell carcinoma
Lung, non small cell carcinoma
Brain, Glioblastoma
Brain, Glioblastoma
Anti-FAK / Focal Adhesion Kinase antibody IHC of human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-FAK / Focal Adhesion Kinase antibody IHC of human Brain, Glioblastoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-FAK / Focal Adhesion Kinase antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-FAK / Focal Adhesion Kinase antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-FAK antibody IHC of human lobular epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-FAK antibody IHC of human lobular epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
See More About...
LSBio Ratings
PathPlus™ FAK / Focal Adhesion Kinase Antibody (C-Terminus) for IHC LS-A2953 has an LSBio Rating of
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit, Chicken
Applications:
IHC, IHC - Paraffin
Species:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit, Chicken
Applications:
IHC, IHC - Paraffin
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Species:
Human
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Species:
Chicken, Human, Rodent
Applications:
Immunofluorescence, Immunoprecipitation
Species:
Human, Mouse, Rat
Applications:
IHC - Paraffin, Western blot
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/16/2024
Date Requested: 4/16/2024
