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Immunohistochemistry Services

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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FAK / Focal Adhesion Kinase

protein tyrosine kinase 2

Non-receptor protein-tyrosine kinase that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis. Required for early embryonic development and placenta development. Required for embryonic angiogenesis, normal cardiomyocyte migration and proliferation, and normal heart development. Regulates axon growth and neuronal cell migration, axon branching and synapse formation; required for normal development of the nervous system. Plays a role in osteogenesis and differentiation of osteoblasts. Functions in integrin signal transduction, but also in signaling downstream of numerous growth factor receptors, G-protein coupled receptors (GPCR), EPHA2, netrin receptors and LDL receptors. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascade. Promotes activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling cascade. Promotes localized and transient activation of guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs), and thereby modulates the activity of Rho family GTPases. Signaling via CAS family members mediates activation of RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ACTN1, ARHGEF7, GRB7, RET and WASL. Promotes phosphorylation of PXN and STAT1; most likely PXN and STAT1 are phosphorylated by a SRC family kinase that is recruited to autophosphorylated PTK2/FAK1, rather than by PTK2/FAK1 itself. Promotes phosphorylation of BCAR1; GIT2 and SHC1; this requires both SRC and PTK2/FAK1. Promotes phosphorylation of BMX and PIK3R1. Isoform 6 (FRNK) does not contain a kinase domain and inhibits PTK2/FAK1 phosphorylation and signaling. Its enhanced expression can attenuate the nuclear accumulation of LPXN and limit its ability to enhance serum response factor (SRF)-dependent gene transcription.

Gene Name: protein tyrosine kinase 2
Family/Subfamily: Protein Kinase , Focal Adhesion Kinase
Synonyms: PTK2, FADK, Fadk1, Focal adhesion kinase, FRNK, FADK 1, Focal adhesion kinase 1, FAK1, p125FAK, PPP1R71, Protein-tyrosine kinase 2, PTK2 protein tyrosine kinase 2, FAK, pp125 FA kinase, Pp125FAK
Target Sequences: NM_005607 NP_005598.3 Q05397

Publications (3)

1
Expression of Src and FAK in hepatocellular carcinoma and the effect of Src inhibitors on hepatocellular carcinoma in vitro. Lau GM, Lau GM, Yu GL, Gelman IH, Gutowski A, Hangauer D, Fang JW. Digestive diseases and sciences. 2009 54:1465-74. (IHC) [PubMed:18979199]
2
Neutrophil granulocytes promote the migratory activity of MDA-MB-468 human breast carcinoma cells via ICAM-1. Strell C, Lang K, Niggemann B, Zaenker KS, Entschladen F. Experimental cell research. 2010 316:138-48. (WB) [PubMed:19747913]
3
Leptin stimulates the migration of colon carcinoma cells by multiple signaling pathways. Ratke J, Entschladen F, Niggemann B, Znker KS, Lang K. Endocrine-related cancer. 2010 17:179-89. (WB) [PubMed:19952122]

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Target
FAK / Focal Adhesion Kinase (228)
Reactivity
Human (207)
Mouse (156)
Rat (136)
Avian (6)
Bat (14)
Bovine (18)
Chicken (46)
Dog (23)
Drosophila (3)
Frog (5)
Guinea pig (2)
Hamster (22)
Horse (13)
Monkey (37)
Rabbit (13)
Rodent (7)
Xenopus (9)
Zebrafish (10)
Application
IHC (96)
IHC-P (54)
WB (179)
Flo (4)
ELISA (63)
ICC (17)
IF (47)
IP (33)
Peptide-ELISA (19)
Host
rabbit (197)
mouse (31)
Isotype
IgG (122)
IgG1 (28)
IgG1,k (2)
IgG2a,k (1)
Clonality
monoclonal mc (32)
polyclonal pc (196)
Clone
10H7 (2)
10H7A6 (2)
10H7E9 (2)
1297CT261.163.145 (1)
12G4 (1)
2C3 (1)
4A9D6 (2)
OTI4A8 (2)
OTI4D11 (2)
OTI4G7 (2)
Format
AP Conjugated (7)
APC (1)
APC Conjugated (8)
Azide-free (5)
Biotin Conjugated (11)
Carrier-free (3)
Concentrated (2)
Cy3 Conjugated (1)
Cy7 Conjugated (1)
FITC Conjugated (11)
HRP Conjugated (11)
PE Conjugated (8)
Unconjugated (170)
Epitope
pTyr397 (18)
Internal (12)
pTyr861 (10)
C-Terminus (9)
pTyr925 (9)
aa426-453 (8)
aa798-1041 (8)
Kinase Domain (7)
pTyr576 (7)
340-420 aa (4)
520-600 aa (4)
aa198-246 (4)
pTyr407 (4)
pSer843 (3)
350-430 aa (2)
780-860 aa (2)
800-880 aa (2)
850-930 aa (2)
860-940 aa (2)
aa706-1052 (2)
pTyr576/Tyr577 (2)
pTyr577 (2)
670-750 aa (1)
Arg919 (1)
Glu393 (1)
Ile401 (1)
Ile855 (1)
Pro906 (1)
Tyr570 (1)
aa1-128 (1)
aa1002-1052 (1)
aa1024-1052 (1)
aa103-553 (1)
aa1036-1052 (1)
aa325-375 (1)
aa364-413 (1)
aa373-422 (1)
aa542-591 (1)
aa543-592 (1)
aa710 (1)
aa748-1053 (1)
aa787-836 (1)
aa809-858 (1)
aa821-848 (1)
aa828-877 (1)
aa876-925 (1)
aa892-941 (1)
pSer576 (1)
pSer732 (1)
pSer910 (1)
pTyr576/577 (1)
Publications
No (228)
Publications
Showing 1-25 of 228 results

IHC-plus™ FAK / Focal Adhesion Kinase Antibody (C-Terminus) LS-A2953
Rabbit Polyclonal to Human FAK / Focal Adhesion Kinase
Mouse, Human, Monkey
IHC, IHC-P
Unconjugated
50µg 50µl 1mg/ml  $425.00
Anti-FAK / Focal Adhesion Kinase antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-FAK antibody IHC of human lobular epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

IHC-plus™ FAK / Focal Adhesion Kinase Antibody (aa706-1052) LS-B7154
Rabbit Polyclonal (IgG1) to Human FAK / Focal Adhesion Kinase
Mouse, Rat, Human
IHC, IHC-P, IP, WB
Unconjugated
50µl  $425.00
Anti-FAK antibody IHC of human colon, lymphocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Western blot Image

IHC-plus™ FAK / Focal Adhesion Kinase Antibody (Internal) LS-A3392
Rabbit Polyclonal to Human FAK / Focal Adhesion Kinase
Chicken, Bovine, Rat, Hamster, Horse, Bat, Rabbit, Mouse, Dog, Human, Monkey
IHC, IHC-P
Unconjugated
50µg 50µl 1mg/ml  $425.00
Immunohistochemistry Image
Anti-FAK antibody IHC of human ductal and lobular epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

FAK / Focal Adhesion Kinase Antibody (aa821-848) LS-C162285
Rabbit Polyclonal to Human FAK / Focal Adhesion Kinase
Human
Flo, IF, IHC, IHC-P, WB
Unconjugated
400µl  $325.00
Formalin-fixed and paraffin-embedded human brain tissue reacted with PTK2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Confocal immunofluorescence of PTK2 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).

FAK / Focal Adhesion Kinase Antibody LS-C497784
Rabbit Monoclonal to Human FAK / Focal Adhesion Kinase
Mouse, Rat, Human
Flo, IF, IHC, IP, WB
Unconjugated
50µl  $295.00; 100µl  $385.00; 200µl  $560.00
Immunohistochemistry of paraffin-embedded mouse spleen using PTK2 antibodyat dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using PTK2 antibodyat dilution of 1:100 (40x lens).

FAK / Focal Adhesion Kinase Antibody (phospho-Tyr925) LS-C177998
Rabbit Polyclonal to Human FAK / Focal Adhesion Kinase
Mouse, Rat, Human
IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $345.00
IHC of p-FAK (Y925) pAb in paraffin-embedded human brain tissue.
Western blot of p-FAK (Y925) pAb in extracts from HepG2 cells.

FAK / Focal Adhesion Kinase Antibody (Internal) LS-C352764
Rabbit Polyclonal to Human FAK / Focal Adhesion Kinase
Chicken, Mouse, Rat, Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $245.00
Immunohistochemical analysis of Focal Adhesion Kinase staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of Focal Adhesion Kinase staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

IHC-plus™ FAK / Focal Adhesion Kinase Antibody (Kinase Domain) LS-A2957
Rabbit Polyclonal to Human FAK / Focal Adhesion Kinase
Chicken, Bovine, Mouse, Dog, Rat, Hamster, Bat, Human, Monkey
IHC, IHC-P
Unconjugated
50µg 50µl 1mg/ml  $425.00
Anti-FAK antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

FAK / Focal Adhesion Kinase Antibody (phospho-Ser910) LS-C198949
Rabbit Polyclonal (IgG) to Human FAK / Focal Adhesion Kinase
Mouse, Rat, Human
IF, IHC, IHC-P, Peptide-ELISA
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunohistochemistry analysis of paraffin-embedded human brain, using FAK (Phospho-Ser910) Antibody. The picture on the right is blocked with the phospho peptide.
Immunofluorescence analysis of HepG2 cells, using FAK (Phospho-Ser910) Antibody. The picture on the right is blocked with the phospho peptide.

FAK / Focal Adhesion Kinase Antibody (phospho-Tyr397) LS-C354970
Rabbit Polyclonal (IgG) to Human FAK / Focal Adhesion Kinase
Xenopus, Chicken, Drosophila, Mouse, Human
IF, IHC, WB
Unconjugated
10blot  $395.00
IF using Phospho-FAK pTyr397 Antibody
WB using Phospho-FAK pTyr397 Antibody

FAK / Focal Adhesion Kinase Antibody (phospho-Tyr925) LS-C352769
Rabbit Polyclonal to Human FAK / Focal Adhesion Kinase
Chicken, Zebrafish, Mouse, Rat, Human
ICC, IF, WB
Unconjugated
100µl 1mg/ml  $265.00
Immunofluorescent analysis of Focal Adhesion Kinase (pY925) staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of Focal Adhesion Kinase (pY925) expression in NIH3T3 pervanadate-treated (A) whole cell lysates.

FAK / Focal Adhesion Kinase Antibody (860-940 aa) LS-C380712
Rabbit Polyclonal (IgG) to Human FAK / Focal Adhesion Kinase
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
50µl 1mg/ml  $235.00; 100µl 1mg/ml  $265.00; 200µl 1mg/ml  $335.00

FAK / Focal Adhesion Kinase Antibody (340-420 aa) LS-C381357
Rabbit Polyclonal (IgG) to Human FAK / Focal Adhesion Kinase
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
50µl 1mg/ml  $235.00; 100µl 1mg/ml  $265.00; 200µl 1mg/ml  $335.00
Western blot of Phospho-FAK (Y397) antibody
Western blot of Phospho-FAK (Y397) antibody

FAK / Focal Adhesion Kinase Antibody LS-C15112
Mouse Monoclonal (IgG1) to Chicken FAK / Focal Adhesion Kinase
Chicken, Rodent, Human
IF, IP
Unconjugated
100µg  $475.00; 200µg  $830.00

FAK / Focal Adhesion Kinase Antibody (phospho-Tyr577) LS-C15122
Rabbit Polyclonal to Human FAK / Focal Adhesion Kinase
Mouse, Human
ELISA, WB
Unconjugated
10blot  $650.00

FAK / Focal Adhesion Kinase Antibody (clone OTI4G7) LS-C174728
Mouse Monoclonal [clone OTI4G7] (IgG1) to Human FAK / Focal Adhesion Kinase
Human
IF, IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $345.00
IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-PTK2 mouse monoclonal antibody.
IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-PTK2 mouse monoclonal antibody.

FAK / Focal Adhesion Kinase Antibody (clone OTI4D11) LS-C174723
Mouse Monoclonal [clone OTI4D11] (IgG1) to Human FAK / Focal Adhesion Kinase
Human
IF, IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $345.00
IHC of paraffin-embedded Human Kidney tissue using anti-PTK2 mouse monoclonal antibody.
IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-PTK2 mouse monoclonal antibody.

FAK / Focal Adhesion Kinase Antibody (clone OTI4A8) LS-C338066
Mouse Monoclonal [clone OTI4A8] (IgG1) to Human FAK / Focal Adhesion Kinase
Human
IF, IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $345.00
IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-PTK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).
IHC of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-PTK2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).

FAK / Focal Adhesion Kinase Antibody (clone 10H7) LS-C758814
Mouse Monoclonal [clone 10H7] (IgG1) to Human FAK / Focal Adhesion Kinase
Mouse, Human
ICC, IF, IHC, WB
Unconjugated
100µl  $425.00
FAK Antibody in Immunohistochemistry (IHC)
FAK Antibody in Immunofluorescence (IF)

FAK / Focal Adhesion Kinase Antibody (clone 10H7E9) LS-C758845
Mouse Monoclonal [clone 10H7E9] (IgG1) to Human FAK / Focal Adhesion Kinase
Human
Flo, ICC, IF, WB
Unconjugated
100µl  $445.00
FAK Antibody in Immunofluorescence (IF)
FAK Antibody in Western Blot (WB)

FAK / Focal Adhesion Kinase Antibody (clone 10H7) LS-C169201
Mouse Monoclonal [clone 10H7] (IgG1) to Human FAK / Focal Adhesion Kinase
Human
ELISA, IF, IHC, WB
Unconjugated
100µl  $345.00
IHC of paraffin-embedded cervices tumor using FAK mouse monoclonal antibody with DAB staining
Immunofluorescence of B16 cells using FAK mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.

IHC-plus™ FAK / Focal Adhesion Kinase Antibody (aa373-422) LS-B12417
Rabbit Polyclonal (IgG) to Human FAK / Focal Adhesion Kinase
Mouse, Rat, Human
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
100µl 1mg/ml  $505.00
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot analysis of lysates from HeLa cells, treated with EGF 200ng/ml 30', using FAK Antibody. The lane on the right is blocked with the synthesized peptide.

FAK / Focal Adhesion Kinase Antibody (C-Terminus) LS-C368815
Rabbit Polyclonal to Human FAK / Focal Adhesion Kinase
Mouse, Human
ICC, IF, IHC, IHC-P, IP, WB
Unconjugated
30µl 1mg/ml  $205.00; 100µl 1mg/ml  $245.00; 200µl 1mg/ml  $305.00
Immunohistochemical analysis of Focal Adhesion Kinase staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected with HRP and DAB as chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of Focal Adhesion Kinase staining in HT29 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

FAK / Focal Adhesion Kinase Antibody LS-C746908
Rabbit Polyclonal (IgG) to Human FAK / Focal Adhesion Kinase
Mouse, Rat, Human
IF, IHC, IP, WB
Unconjugated
50µl  $235.00; 100µl  $295.00; 200µl  $385.00
Immunohistochemistry of paraffin-embedded human colon using PTK2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using PTK2 antibody at dilution of 1:100 (40x lens).

IHC-plus™ FAK / Focal Adhesion Kinase Antibody (phospho-Tyr576) LS-B2522
Rabbit Polyclonal to Human FAK / Focal Adhesion Kinase
Frog, Chicken, Mouse, Rat, Human
ELISA, IHC, IHC-P, WB
Unconjugated
10blot  $605.00
Anti-FAK antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Showing 1-25 of 228 results

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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).