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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Collagen II Antibody (clone 2B1.5) LS‑C87481
Note: This antibody replaces LS-C79524
Collagen II antibody LS-C87481 is an unconjugated mouse monoclonal antibody to Collagen II from human, mouse, rat and other species. Validated for Flow, IF, IHC and WB.
Catalog
Size
Price
LS-C87481-100
100 µl
Unavailable
LS-C87481-500
500 µl
Unavailable
LS-C87481-1000
1000 µl
Unavailable

Popular Collagen II Products

Antibody
Species: Human, Bovine
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Anti-Collagen II Antibody - Immunohistochemistry. anti-collagen II antibody (1:400, 45 min RT) showed moderate staining of in FFPE human bronchiolar cartilage (shown in image). Though not shown, faint to moderate staining of tonsillar squamous epithelium, prostatic stroma, breast, colon, placenta, and dermal connective tissues was also observed. All other tissues, including brain, breast epithelium, colon epithelium, heart, intestine, kidney, liver, lung, skeletal muscle, pancreas, spleen, testis, thymus, thyroid, and uterus were negative for staining Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval. Image provided courtesy of LifeSpan Biosciences, Seattle, WA.
Species: Human, Mouse, Rat, Bovine, Sheep
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Formalin-fixed, paraffin-embedded human lung stained with peroxidase-conjugate and AEC chromogen. Note staining of matrix in the bronchial cartilaginous plates.  This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Chicken, Human, Mouse, Rat, Bovine
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Formalin-fixed, paraffin-embedded human fetus stained with peroxidase-conjugate and AEC chromogen. Note staining of matrix in the cartilaginous plates.  This image was taken for the unmodified form of this product. Other forms have not been tested.
Species: Chicken, Human, Mouse, Salamander
Applications: IHC, IHC - Paraffin, Immunofluorescence

Product Description

Collagen II antibody LS-C87481 is an unconjugated mouse monoclonal antibody to Collagen II from human, mouse, rat and other species. Validated for Flow, IF, IHC and WB.
About Collagen II
Type II collagen is the basis for articular cartilage and hyaline cartilage. It makes up 50% of all protein in cartilage and 85-90% of collagen of articular cartilage. Type II collagen does form fibrils. This fibrillar network of collagen allows cartilage to entrap the proteoglycan aggregate as well as provide tensile strength to the tissue.

Specifications

Target
Chicken Collagen II
Host
Mouse
Reactivity
Chicken, Human, Mouse, Rat, Bovine (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal [2B1.5]
Conjugations
Unconjugated. Also available conjugated with Biotin.
Purification
Protein A purified
Modifications
Unmodified. Also available Azide-free.
Applications
  • IHC
  • IHC - Paraffin (1 - 2 µg/ml)
  • Immunofluorescence
  • Western blot (1 - 2 µg/ml)
  • Flow Cytometry
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Collagen II antibody was raised against a purified preparation of lathyritic type II collagen from embryonic chicken sternum.
Specificity
Carboxyl-terminal one quarter of the type II collagen. The epitope for 2B1.5 MAb is not susceptible to digestion of lathyritic type II collagen with recombinant stromelysin.
Usage
Staining of FFPE tissues requires digestion of tissue sections with pepsin at 1 mg/ml in Tris-HCl pH 2.0 for 15 minutes at room temperature or 10 minutes at 37C.
Presentation
10mM PBS, pH 7.4, with 0.2% BSA and 0.09% Sodium Azide
Storage
Stable for 2 years when stored at 2-8°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Formalin-fixed, paraffin-embedded human lung stained with peroxidase-conjugate and AEC chromogen. Note staining of matrix in the bronchial cartilaginous plates.  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human lung stained with peroxidase-conjugate and AEC chromogen. Note staining of matrix in the bronchial cartilaginous plates. This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry

Formalin-fixed, paraffin-embedded human lung stained with peroxidase-conjugate and AEC chromogen. Note staining of matrix in the bronchial cartilaginous plates.  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human lung stained with peroxidase-conjugate and AEC chromogen. Note staining of matrix in the bronchial cartilaginous plates. This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry

Formalin-fixed, paraffin-embedded human lung stained with peroxidase-conjugate and AEC chromogen. Note staining of matrix in the bronchial cartilaginous plates.  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human lung stained with peroxidase-conjugate and AEC chromogen. Note staining of matrix in the bronchial cartilaginous plates. This image was taken for the unmodified form of this product. Other forms have not been tested.

Immunohistochemistry

Formalin-fixed, paraffin-embedded human lung stained with peroxidase-conjugate and AEC chromogen. Note staining of matrix in the bronchial cartilaginous plates.  This image was taken for the unmodified form of this product. Other forms have not been tested.
Formalin-fixed, paraffin-embedded human lung stained with peroxidase-conjugate and AEC chromogen. Note staining of matrix in the bronchial cartilaginous plates. This image was taken for the unmodified form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/25/2018

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