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Catalog Number Size Price
LS-C745279-25 25 µl (1 mg/ml) $275 
Collagen II Antibody - Anti collagen II antibody showed moderate staining of in FFPE human bronchiolar cartilage (shown in image). Though not shown, faint to moderate staining of tonsillar squamous epithelium, prostatic stroma, breast, colon, placenta, and dermal connective tissues was also observed. All other tissues, including brain, breast epithelium, colon epithelium, heart, intestine, kidney, liver, lung, skeletal muscle, pancreas, spleen, testis, thymus, thyroid, and uterus were negative for staining Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes for antigen retrieval.

Polyclonal Rabbit anti‑Mammal Collagen II Antibody (IHC, WB) LS‑C745279

Polyclonal Rabbit anti‑Mammal Collagen II Antibody (IHC, WB) LS‑C745279

Antibody:
Collagen II Rabbit anti-Mammal Polyclonal Antibody
Application:
IHC, WB, IP, ELISA
Reactivity:
Mammal, Human, Mouse, Rat, Bovine, Sheep
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C745279-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Collagen II Rabbit anti-Mammal Polyclonal Antibody
Application:
IHC, WB, IP, ELISA
Reactivity:
Mammal, Human, Mouse, Rat, Bovine, Sheep
Format:
Unconjugated, Unmodified

Specifications

Description
Collagen II antibody LS-C745279 is an unconjugated rabbit polyclonal antibody to Collagen II from mammal. It is reactive with human, mouse, rat and other species. Validated for ELISA, IHC, IP and WB.
Target
Mammal Collagen II
Host
Rabbit
Reactivity
Mammal, Human, Mouse, Rat, Bovine, Sheep (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
Collagen Type II from human knee cartilage and bovine nasal cartilage.
Specificity
Typically negligible cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type II collagens and has negligible cross-reactivity with Type I, III, IV, V or VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Applications
  • IHC (1:50 - 1:400)
  • Western blot (1:1000 - 1:10000)
  • Immunoprecipitation (1:100)
  • ELISA (1:5000 - 1:50000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/12/2021