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Catalog Number Size Price
LS-C18866-100 100 µg (1 mg/ml) $504 
Collagen II Antibody - Immunohistochemistry of Rabbit Anti-Collagen II Biotin Conjugated Antibody. Tissue: human bronchiolar cartilage (shown). Though not shown, faint to moderate staining of tonsillar squamous epithelium, prostatic stroma, breast, colon, placenta, and dermal connective tissues was also observed. All other tissues, including brain, breast epithelium, colon epithelium, heart, intestine, kidney, liver, lung, skeletal muscle, pancreas, spleen, testis, thymus, thyroid, and uterus were negative for staining. Fixation: formalin fixed paraffin embedded. Antigen retrieval: 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes. Primary antibody: collagen II antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Collagen II is extracellular. Staining: Collagen II as precipitated red signal with hematoxylin purple nuclear counterstain.

Polyclonal Rabbit anti‑Mammal Collagen II Antibody (Biotin, IHC, WB) LS‑C18866

Polyclonal Rabbit anti‑Mammal Collagen II Antibody (Biotin, IHC, WB) LS‑C18866

Antibody:
Collagen II Rabbit anti-Mammal Polyclonal (Biotin) Antibody
Application:
IHC, IHC-P, WB, IP, ELISA
Reactivity:
Mammal, Human, Bovine
Format:
Biotin, Unmodified
Price
Catalog Number
$504
LS-C18866-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
Collagen II Rabbit anti-Mammal Polyclonal (Biotin) Antibody
Application:
IHC, IHC-P, WB, IP, ELISA
Reactivity:
Mammal, Human, Bovine
Format:
Biotin, Unmodified

Specifications

Description
Collagen II antibody LS-C18866 is a biotin-conjugated rabbit polyclonal antibody to Collagen II from mammal. It is reactive with human, bovine and mammal. Validated for ELISA, IHC, IP and WB.
Target
Mammal Collagen II
Host
Rabbit
Reactivity
Mammal, Human, Bovine (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Biotin
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
Anti-Collagen Type II antibody was produced by repeated immunizations with Collagen Type II from adult human knee cartilage and bovine nasal cartilage.
Specificity
Typically negligible cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type II collagens and has negligible cross-reactivity with Type I, III, IV, V and VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Applications
  • IHC
  • IHC - Paraffin
  • Western blot (1:5000 - 1:10000)
  • Immunoprecipitation (1:100)
  • ELISA (1:10000 - 1:50000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Store lyophilized at 4°C. Following resuspension aliquot and store at -20°C. Avoid freeze-thaw cycles and dilute immediately before use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/10/2022