Mammal Collagen II
Mammal, Human, Bovine
(tested or 100% immunogen sequence identity)
Anti-Collagen Type II antibody was produced by repeated immunizations with Collagen Type II from adult human knee cartilage and bovine nasal cartilage.
Typically negligible cross reactivity against other types of collagens was detected by ELISA against purified standards. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type II collagens and has negligible cross-reactivity with Type I, III, IV, V and VI collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
- IHC - Paraffin
- Western blot (1:5000 - 1:10000)
- Immunoprecipitation (1:100)
- ELISA (1:10000 - 1:50000)
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Used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitute in 100 µL of deionized water.
Store lyophilized at 4°C. Following resuspension aliquot and store at -20°C. Avoid freeze-thaw cycles and dilute immediately before use.
For research use only. Intended for use by laboratory professionals.
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