Mouse Collagen II

Rabbit

Mouse, Cow (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated

Unmodified

Purified collagen type II from fetal murine cartilage

• IHC - Paraffin (1:1000)
• Immunofluorescence (1:80)
• Western blot
• ELISA (1:200)
• Radioimmunoassay

After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg) in TBS 15 minutes at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 minutes at RT is recommended. Type II collagen is an alpha1(II)-trimer, which forms 67 nm cross-banded fibrils. Typically it can be observed in cartilage and various tumours. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Murine collagen type II 100%, murine collagen type I, III and IV <0.1%, Mouse fibronectin < 0.1%.

PBS

0.5 ml Reconstitute in sterile distilled water.

Store at -20°C.

For research use only. Intended for use by laboratory professionals.

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