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Anti-BRAF / B-Raf Antibody (phospho-Ser602) LS-C198695

Catalog Size Price
LS-C198695-50 50 µl Unavailable
LS-C198695-100 100 µl Unavailable

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100% Guaranteed
Rabbit Polyclonal (IgG) to Human BRAF / B-Raf
Human, Mouse, Rat
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Unconjugated

Details

Human BRAF / B-Raf
Rabbit
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:500 - 1:1000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000)

Specificity and Use

BRAF / B-Raf antibody was raised against synthetic peptide derived from human B-RAF around the phosphorylation site of Ser602.
pSer602
B-RAF (Phospho-Ser602) Antibody detects endogenous levels of B-RAF only when phosphorylated at serine602.

Packaging

PBS, pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
Store at -20°C.
For research use only.

About BRAF / B-Raf

P15056 NM_004333 NP_004324.2

BRAF Antibody, 94 kDa B-raf protein Antibody, B-raf Antibody, BRAF1 Antibody, B Raf Antibody, Proto-oncogene B-Raf Antibody, p94 Antibody, B-RAF1 Antibody, NS7 Antibody, ONCOGENE BRAF Antibody, RAFB1 Antibody

BRAF / B-Raf is a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance.

Immunohistochemistry

IHC of paraffin-embedded human lymph node, using B-RAF (Phospho-Ser602) Antibody. The sample on the right was incubated with synthetic peptide.
IHC of paraffin-embedded human lymph node, using B-RAF (Phospho-Ser602) Antibody. The sample on the right was incubated with synthetic peptide.

Western blot

Western blot of extracts from NIH-3T3 cells treated with EGF 200ng/ml 30', using B-RAF (Phospho-Ser602) Antibody. The lane on the right was incubated with synthetic peptide.
Western blot of extracts from NIH-3T3 cells treated with EGF 200ng/ml 30', using B-RAF (Phospho-Ser602) Antibody. The lane on the right was incubated with synthetic peptide.

Requested From: 
Date Requested: 5/21/2018

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