LSBio
LSBio
Products
Services
Research Areas
Resources
Contact Us
Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)

How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Products
Services
Research Areas
Resources
Contact Us

BRAF / B-Raf Antibody (clone 3A7) LS-C114543

IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Ovary tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded breast tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded colon tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded pancreas tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
Anti-BRAF mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BRAF.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BRAF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BRAF.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-anti-BRAF monoclonal antibody.
IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded Ovary tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded breast tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded colon tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded pancreas tissue using anti-BRAF mouse monoclonal antibody. (Dilution 1:50).
Anti-BRAF mouse monoclonal antibody  immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BRAF.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BRAF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BRAF.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-anti-BRAF monoclonal antibody.
1 of 8
2 of 8
3 of 8
4 of 8
5 of 8
6 of 8
7 of 8
8 of 8

BRAF / B-Raf Antibody (clone 3A7) LS-C114543

Antibody:
Mouse Monoclonal to Human BRAF / B-Raf
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human, Monkey, Rat, Dog
Format:
Unconjugated, Unmodified
Price
Catalog Number
$345 
LS-C114543-100
Toll Free North America
866-819-4732

Overview

Antibody:
Mouse Monoclonal to Human BRAF / B-Raf
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human, Monkey, Rat, Dog
Format:
Unconjugated, Unmodified

Specifications

Description
B-Raf antibody LS-C114543 is an unconjugated mouse monoclonal antibody to B-Raf (BRAF) from human, rat, dog and other species. Validated for IF, IHC and WB.

Target
Human BRAF / B-Raf
Host
Mouse
Reactivity
Human, Monkey, Rat, Dog (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal
Clone
3A7
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50)
  • Immunofluorescence (1:100)
  • Western blot (1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
BRAF / B-Raf antibody was raised against protein expressed in 293T cell transfected with human BRAF expression vector.
Specificity
Human BRAF / B-Raf
Usage
Concentration: 0.5-1.0 mg/ml (Lot dependent)
Presentation
PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol, 1% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BRAF / B-Raf
P15056 NM_004333 NP_004324.2

Publications (0)

Reviews (0)

Featured Products

Reactivity: Mouse
Range: 7.8-500 pg/ml
Reactivity: Human
Range: 0.78-50 ng/ml
Reactivity: Human
Range: 0.156-10 ng/ml
Reactivity: Human
Range: 15.6-1000 pg/ml
Anti-ELANE / Neutrophil Elastase antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, ELISA, Immunoelectrophoresis

Requested From: United States
Date Requested: 7/19/2019
Get Social With Us!
Follow us on Facebook Follow us on LinkedIn
Copyright © 2019 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy