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Anti-BCL2 / Bcl-2 Antibody (Thr56) IHC-plus™ LS-B1173

Note: This antibody replaces LS-C34295, LS-C74703, LS-C51195, LS-C118063

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular BCL2 / Bcl-2 Antibodies

Anti-BCL2 / Bcl-2 Antibody (clone E17) LS-C202809
Rabbit Monoclonal [clone E17] (IgG) to Human BCL2 / Bcl-2
Human
IHC - Paraffin, IHC - Frozen
Unconjugated
Anti-BCL2 / Bcl-2 Antibody (C-Terminus) IHC-plus™ LS-B11858
Rabbit Monoclonal (IgG) to Human BCL2 / Bcl-2
Human, Monkey, Mouse, Rat, Bovine
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human BCL2 / Bcl-2
Human
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated

Details

Human BCL2 / Bcl-2
Rabbit
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (20 µg/ml)
  • Immunofluorescence
  • Western blot

Specificity and Use

BCL2 / Bcl-2 antibody was raised against synthetic peptide from human BCL2 / Bcl-2.
Thr56
Amino acids surrounding Threonine 56 of human BCL2
Immunohistochemistry: LS-B1173 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1173 was determined to be 20 ug/ml.

Packaging

PBS, 0.02% sodium azide, 50% glycerol.
Long term: -70°C; Short term: -20°C
For research use only.

About BCL2 / Bcl-2

P10415 NM_000633 NP_000624.2

BCL2 Antibody, Apoptosis regulator Bcl-2 Antibody, B-cell CLL/lymphoma 2 Antibody, B-cell lymphoma protein 2 Antibody, Bcl-2 Antibody, PPP1R50 Antibody

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).

Immunohistochemistry

Immunohistochemistry
Anti-BCL2 / Bcl-2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1173 concentration 20 ug/ml.

Immunofluorescence

Immunofluorescence
Immunofluorescence staining of methanol-fixed HeLa cells using BCL-2(Ab-56) antibody.

Western blot

Western blot
Western blot of extracts from HeLa, A2780 and 293 cells using BCL-2(Ab-56) antibody.
 

Requested From: United States
Date Requested: 12/6/2016

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