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Anti-AKT1 + AKT2 + AKT3 Antibody (phospho-Tyr315/Tyr316/Tyr312) LS-C205291

Ordering

Wt. Vol. Conc. Price
- 50 µl 1 mg/ml $285
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Most Popular AKT1 + AKT2 + AKT3 Antibodies

Anti-AKT1 + AKT2 + AKT3 Antibody (C-Terminus) IHC-plus™ LS-B2844
Rabbit Polyclonal to Human AKT1 + AKT2 + AKT3
Human, Mouse, Rat, Chicken
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunohistochemistry Image
Anti-AKT1 + AKT2 + AKT3 Antibody (Internal) LS-C354521
Rabbit Polyclonal to Human AKT1 + AKT2 + AKT3
Human, Mouse, Rat, Bovine, Sheep
IHC - Paraffin, ICC, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image

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Rabbit Polyclonal to Human AKT1 + AKT2 + AKT3
Human, Mouse, Rat
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated

Details

Human AKT1 + AKT2 + AKT3
Rabbit
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:100 - 1:200)
  • Western blot (1:500 - 1:1000)

Specificity and Use

Peptide sequence around phosphorylation site of tyrosine 315/316/312 (P-E-Y (p) -L-A) of human AKT1/AKT2/AKT3.
pTyr315/Tyr316/Tyr312
The antibody detects endogenous levels of AKT1/AKT2/AKT3 only when phosphorylated at tyrosine 315/316/312.

Packaging

PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
For research use only.

About AKT1 + AKT2 + AKT3

AKT, also known as protein kinase B (PKB), constitutes a family of serine/threonine kinases. AKT has 3 isoforms: AKT1, AKT2), and AKT3). These isoforms share a high degree of homology, especially in the catalytic domains (87%-90% identical amino acids).

Immunohistochemistry

Immunohistochemistry
Immunohistochemistry of paraffin-embedded human breast carcinoma tissue using AKT1/AKT2/AKT3 (Phospho-Tyr315/316/312) antibody (left) or the same antibody preincubated with blocking peptide (right).

Immunofluorescence

Immunofluorescence
Immunofluorescence of methanol-fixed HeLa cells using AKT1/AKT2/AKT3 (phospho-Tyr315/316/312) antibody.

Western blot

Western blot
Western blot of extracts from HepG2 cells untreated or treated with EGF using AKT1/AKT2/AKT3 (phospho-Tyr315/316/312) antibody.
 

Requested From: United States
Date Requested: 12/7/2016

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