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Catalog Number Size Price
LS-B2844-50 50 µl (85 mg/ml) $515 
AKT1 + AKT2 + AKT3 Antibody - Anti-AKT1/2/3 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
AKT1 + AKT2 + AKT3 Antibody - Immunofluorescence Microscopy of Rabbit Anti-AKT Antibody. Tissue: neonatal rat cardiomyocytes. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: AKT antibody at 1:80 dilution for 1 h at RT. Secondary antibody: Texas-red conjugated rabbit secondary antibody at 1:10000 for 45 min at RT. Localization: AKT is nuclear. Staining: Anti-AKT staining appears green. Actin filaments are labeled red using a Texas-red conjugated phalloidin.
AKT1 + AKT2 + AKT3 Antibody - Immunofluorescence microscopy. Rabbit anti-AKT was used at a 1:80 dilution to stain cultured neonatal rat cardiomyocytes that express a nuclear-targeted AKT construct. Anti-AKT staining appears green. Actin filaments are labeled red using a Texas-red conjugated phalloidin.
AKT1 + AKT2 + AKT3 Antibody - Western blot of Rabbit Anti-AKT antibody. Lane 1: NIH/3T3 whole cell lysate. Load: 20 ug per lane. Primary antibody: AKT antibody at 1:500 for overnight at 4C. Secondary antibody: HRP conjugated Gt-a-Rabbit IgG (LS-C60865) at 1:10000 preceded color development using Pierce Chemical's SuperSignal substrate. Block: MOPS buffer overnight at 4C. Predicted/Observed size: 128 kDa, 128 kDa for AKT. Other band(s): none.
AKT1 + AKT2 + AKT3 Antibody - Western Blot. Rabbit anti-AKT was used at a 1:500 dilution to detect AKT by Western blot.
AKT1 + AKT2 + AKT3 Antibody - Anti-AKT1/2/3 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
AKT1 + AKT2 + AKT3 Antibody - Immunofluorescence Microscopy of Rabbit Anti-AKT Antibody. Tissue: neonatal rat cardiomyocytes. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: AKT antibody at 1:80 dilution for 1 h at RT. Secondary antibody: Texas-red conjugated rabbit secondary antibody at 1:10000 for 45 min at RT. Localization: AKT is nuclear. Staining: Anti-AKT staining appears green. Actin filaments are labeled red using a Texas-red conjugated phalloidin.
AKT1 + AKT2 + AKT3 Antibody - Immunofluorescence microscopy. Rabbit anti-AKT was used at a 1:80 dilution to stain cultured neonatal rat cardiomyocytes that express a nuclear-targeted AKT construct. Anti-AKT staining appears green. Actin filaments are labeled red using a Texas-red conjugated phalloidin.
AKT1 + AKT2 + AKT3 Antibody - Western blot of Rabbit Anti-AKT antibody. Lane 1: NIH/3T3 whole cell lysate. Load: 20 ug per lane. Primary antibody: AKT antibody at 1:500 for overnight at 4C. Secondary antibody: HRP conjugated Gt-a-Rabbit IgG (LS-C60865) at 1:10000 preceded color development using Pierce Chemical's SuperSignal substrate. Block: MOPS buffer overnight at 4C. Predicted/Observed size: 128 kDa, 128 kDa for AKT. Other band(s): none.
AKT1 + AKT2 + AKT3 Antibody - Western Blot. Rabbit anti-AKT was used at a 1:500 dilution to detect AKT by Western blot.
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IHC‑plus™ Polyclonal Rabbit anti‑Human AKT1 + AKT2 + AKT3 Antibody (aa460‑480, IHC, IF, WB) LS‑B2844

IHC‑plus™ Polyclonal Rabbit anti‑Human AKT1 + AKT2 + AKT3 Antibody (aa460‑480, IHC, IF, WB) LS‑B2844

Note: This antibody replaces LS-C18882
Antibody:
AKT1 + AKT2 + AKT3 Rabbit anti-Human Polyclonal (aa460-480) Antibody
Application:
IHC, IHC-P, ICC, IF, WB, Flo, ELISA
Reactivity:
Human, Mouse, Rat, Chicken
Format:
Unconjugated, Unmodified
Price
Catalog Number
$515
LS-B2844-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
AKT1 + AKT2 + AKT3 Rabbit anti-Human Polyclonal (aa460-480) Antibody
Application:
IHC, IHC-P, ICC, IF, WB, Flo, ELISA
Reactivity:
Human, Mouse, Rat, Chicken
Format:
Unconjugated, Unmodified

Specifications

Description
AKT1 + AKT2 + AKT3 antibody LS-B2844 is an unconjugated rabbit polyclonal antibody to AKT1 + AKT2 + AKT3 (aa460-480) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, Flow, ICC, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human AKT1 + AKT2 + AKT3
Host
Rabbit
Reactivity
Human, Mouse, Rat, Chicken (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Delipidated and defibrinated
Modifications
Unmodified
Immunogen
AKT Antibody was produced from whole rabbit serum prepared by repeated immunizations with a synthetic peptide R-P-H-F-P-Q-F-S-Y-S-A-S-G-T-A corresponding to the C-terminus (460-480) of human AKT proteins conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling. A BLAST analysis was used to suggest reactivity with this protein from rat, mouse, and chicken based on 100% homology for the immunogen sequence.
Epitope
aa460-480
Specificity
Reacts with the AKT from human tissues. Based on sequence we expect this antibody to react as well with rat, mouse, and chicken AKT.
Applications
  • IHC
  • IHC - Paraffin (1:100)
  • ICC
  • Immunofluorescence (1:100 - 1:1000)
  • Western blot (1:500 - 1:2000)
  • Flow Cytometry
  • ELISA (1:2000 - 1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Immunohistochemistry: LS-B2844 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2844 was determined to be 1:100.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid freeze/thaw cycles. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

Validation

AKT1 + AKT2 + AKT3 Antibody - Anti-AKT1/2/3 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-AKT1/2/3 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Anti-AKT1/2/3 antibody IHC of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.

LSBio Ratings

IHC-plus™ AKT1 + AKT2 + AKT3 Antibody (aa460-480) for IHC, ICC, IF/Immunofluorescence, WB/Western, Flow, ELISA LS-B2844 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)

Publications (1)

Silencing of caspase-8 and caspase-3 by RNA interference prevents vascular endothelial cell injury in mice with endotoxic shock. Matsuda N, Takano Y, Kageyama S, Hatakeyama N, Shakunaga K, Kitajima I, Yamazaki M, Hattori Y. Cardiovascular research. 2007 76:132-40.

Customer Reviews (0)


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Requested From: United States
Date Requested: 6/20/2024