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Catalog Number Size Price
LS-C745285-25 25 µl (1 mg/ml) $275 
AKT1 + AKT2 + AKT3 Antibody - Immunofluorescence Confocal Microscopy of rabbit anti-AKT pS473 antibody. Tissue: cardiomyocytes infected with adenovirus expressing with wild-type AKT. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: AKT pS473 antibody at 1:40 for 1 h at RT. Secondary antibody: texas-red conjugated rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: AKT pS473 is nuclear. Staining: AKT pS473 as green fluorescent signal with texas-red conjugated phalloidin (red) to label filamentous actin.
AKT1 + AKT2 + AKT3 Antibody - Immunohistochemistry of rabbit anti-AKT pS473 antibody. Tissue: human breast carcinoma. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: AKT pS473 antibody at 1:100 for 1 h at RT. Secondary antibody: Dako's Techmate streptavidin-biotin reagents at 1:10,000 for 45 min at RT. Localization: AKT pS473 is nuclear and occasionally cytoplasmic.
AKT1 + AKT2 + AKT3 Antibody - Immunohistochemistry of rabbit Anti-Akt pS473 antibody. Tissue: human breast carcinoma. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Akt pS473 antibody at 100 dilution for 1 h at RT. Secondary antibody: Dako's Techmate streptavidin-biotin reagents at 1:10,000 for 45 min at RT. Localization: Akt pS473 is nuclear and occasionally cytoplasmic.
AKT1 + AKT2 + AKT3 Antibody - Immunohistochemistry at higher magnification of rabbit Anti-Akt pS473 antibody. Tissue: human breast carcinoma. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Akt pS473 antibody at 100 dilution for 1 h at RT. Secondary antibody: Dako's Techmate streptavidin-biotin reagents at 1:10,000 for 45 min at RT. Localization: Akt pS473 is nuclear and occasionally cytoplasmic.
AKT1 + AKT2 + AKT3 Antibody - Western Blot of rabbit anti-AKT pS473 antibody. Lane 1: AKT1 Recombinant Protein Lane 2: AKT1 Mutant Human Recombinant Protein Lane 3: AKT1 (phosphatase treated) Human Recombinant Protein Load: 50 ng per lane. Primary antibody: AKT pS473 antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting (MB-070) for 30 min at RT. Predicted/Observed size: ~56 kDa for AKTpS473.
AKT1 + AKT2 + AKT3 Antibody - Immunofluorescence Confocal Microscopy of rabbit anti-AKT pS473 antibody. Tissue: cardiomyocytes infected with adenovirus expressing with wild-type AKT. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: AKT pS473 antibody at 1:40 for 1 h at RT. Secondary antibody: texas-red conjugated rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: AKT pS473 is nuclear. Staining: AKT pS473 as green fluorescent signal with texas-red conjugated phalloidin (red) to label filamentous actin.
AKT1 + AKT2 + AKT3 Antibody - Immunohistochemistry of rabbit anti-AKT pS473 antibody. Tissue: human breast carcinoma. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: AKT pS473 antibody at 1:100 for 1 h at RT. Secondary antibody: Dako's Techmate streptavidin-biotin reagents at 1:10,000 for 45 min at RT. Localization: AKT pS473 is nuclear and occasionally cytoplasmic.
AKT1 + AKT2 + AKT3 Antibody - Immunohistochemistry of rabbit Anti-Akt pS473 antibody. Tissue: human breast carcinoma. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Akt pS473 antibody at 100 dilution for 1 h at RT. Secondary antibody: Dako's Techmate streptavidin-biotin reagents at 1:10,000 for 45 min at RT. Localization: Akt pS473 is nuclear and occasionally cytoplasmic.
AKT1 + AKT2 + AKT3 Antibody - Immunohistochemistry at higher magnification of rabbit Anti-Akt pS473 antibody. Tissue: human breast carcinoma. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: Akt pS473 antibody at 100 dilution for 1 h at RT. Secondary antibody: Dako's Techmate streptavidin-biotin reagents at 1:10,000 for 45 min at RT. Localization: Akt pS473 is nuclear and occasionally cytoplasmic.
AKT1 + AKT2 + AKT3 Antibody - Western Blot of rabbit anti-AKT pS473 antibody. Lane 1: AKT1 Recombinant Protein Lane 2: AKT1 Mutant Human Recombinant Protein Lane 3: AKT1 (phosphatase treated) Human Recombinant Protein Load: 50 ng per lane. Primary antibody: AKT pS473 antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting (MB-070) for 30 min at RT. Predicted/Observed size: ~56 kDa for AKTpS473.
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Polyclonal Rabbit anti‑Human AKT1 + AKT2 + AKT3 Antibody (phospho‑Ser473, IHC, IF, WB) LS‑C745285

Polyclonal Rabbit anti‑Human AKT1 + AKT2 + AKT3 Antibody (phospho‑Ser473, IHC, IF, WB) LS‑C745285

Antibody:
AKT1 + AKT2 + AKT3 Rabbit anti-Human Polyclonal (pSer473) Antibody
Application:
IHC, IF, WB, Flo, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C745285-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
AKT1 + AKT2 + AKT3 Rabbit anti-Human Polyclonal (pSer473) Antibody
Application:
IHC, IF, WB, Flo, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
AKT1 + AKT2 + AKT3 antibody LS-C745285 is an unconjugated rabbit polyclonal antibody to AKT1 + AKT2 + AKT3 (pSer473) from human. It is reactive with human, mouse and rat. Validated for ELISA, Flow, IF, IHC and WB.
Target
Human AKT1 + AKT2 + AKT3
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity chromatography
Modifications
Unmodified
Immunogen
Rabbit Anti-AKTpS473 Antibody was prepared by repeated immunizations in rabbits with a synthetic peptide corresponding to a C-terminus region near phospho Serine 473 of the human, mouse, rat and chicken AKT proteins conjugated to KLH.
Epitope
pSer473
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. This antibody is specific for phosphorylated human AKTpS473. Minimal reactivity occurs against non-phosphorylated AKT. Reactivity against AKT from other species may occur but has not yet been tested.
Applications
  • IHC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:200 - 1:1000)
  • Flow Cytometry
  • ELISA (1:15000 - 1:60000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/14/2021