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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Actin Antibody (N‑Terminus, clone EP184E) IHC‑plus™ LS‑B6276
Note: This antibody replaces LS-C49807
Actin antibody LS-B6276 is an unconjugated rabbit monoclonal antibody to Actin from human, mouse and rat. Validated for Flow, ICC, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B6276-50
50 µl (0.156 mg/ml)
Unavailable

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IHC of paraffin-embedded human skeletal muscle tissue, using Actin-pan ( alpha/gamma) (Ab-55/53) Antibody. The picture on the right is treated with the synthesized peptide.
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 This image was taken for the unconjugated form of this product. Other forms have not been tested.
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 This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Applications: Immunoprecipitation

Product Description

Actin antibody LS-B6276 is an unconjugated rabbit monoclonal antibody to Actin from human, mouse and rat. Validated for Flow, ICC, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.

Specifications

Target
Human Actin
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Monoclonal [EP184E]
Conjugations
Unconjugated
Purification
Tissue culture supernatant
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:200)
  • ICC (1:250 - 1:500)
  • Western blot (1:1000)
  • Immunoprecipitation (1:20)
  • Flow Cytometry (1:20)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Actin antibody was raised against a synthetic peptide corresponding to residues near the N-terminus of human Actin.
Epitope
N-Terminus
Presentation
Tris-Glycine-NaCl or PBS, pH 7.2-7.4, 40-50% Glycerol, 0.01% sodium azide, 0.05% BSA
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-ACTC1 / Alpha-Cardiac Actin antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6276 dilution 1:50.
Anti-ACTC1 / Alpha-Cardiac Actin antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6276 dilution 1:50.

Immunocytochemistry

Immunofluorescent staining of HeLa cells using anti-Actin antibody.
Immunofluorescent staining of HeLa cells using anti-Actin antibody.

Western blot

Western blot analysis on A431 cell lysate using anti-Actin antibody at 1:1000.
Western blot analysis on A431 cell lysate using anti-Actin antibody at 1:1000.

Immunohistochemistry

Anti-ACTC1 / Alpha-Cardiac Actin antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6276 dilution 1:50.
Anti-ACTC1 / Alpha-Cardiac Actin antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6276 dilution 1:50.

Immunocytochemistry

Immunofluorescent staining of HeLa cells using anti-Actin antibody.
Immunofluorescent staining of HeLa cells using anti-Actin antibody.

Western blot

Western blot analysis on A431 cell lysate using anti-Actin antibody at 1:1000.
Western blot analysis on A431 cell lysate using anti-Actin antibody at 1:1000.

Immunohistochemistry

Anti-ACTC1 / Alpha-Cardiac Actin antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6276 dilution 1:50.
Anti-ACTC1 / Alpha-Cardiac Actin antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6276 dilution 1:50.

Immunocytochemistry

Immunofluorescent staining of HeLa cells using anti-Actin antibody.
Immunofluorescent staining of HeLa cells using anti-Actin antibody.

Western blot

Western blot analysis on A431 cell lysate using anti-Actin antibody at 1:1000.
Western blot analysis on A431 cell lysate using anti-Actin antibody at 1:1000.

Immunohistochemistry

Anti-ACTC1 / Alpha-Cardiac Actin antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6276 dilution 1:50.
Anti-ACTC1 / Alpha-Cardiac Actin antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6276 dilution 1:50.

Immunocytochemistry

Immunofluorescent staining of HeLa cells using anti-Actin antibody.
Immunofluorescent staining of HeLa cells using anti-Actin antibody.

Western blot

Western blot analysis on A431 cell lysate using anti-Actin antibody at 1:1000.
Western blot analysis on A431 cell lysate using anti-Actin antibody at 1:1000.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.


Requested From: 
Date Requested: 6/24/2018

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