Products
Research Areas
COVID-19
Resources
Contact Us
Distributors
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Contact Us
LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121
Phone:
866-819-4732 (Toll Free North America
206-374-1102 (International)
Fax:
866-206-6909 (Toll Free North America)
206-577-4565 (International)
How To Buy - Details about how to buy our products.
Orders@LSBio.com - To submit a new order.
Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, Proforma invoice requests, or other billing issues.
Technical.Support@LSBio.com - To request technical information about an LSBio product or its applications
Sales@LSBio.com - To request information about distribution agreements, or general business development.
Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Catalog Number Size Price
LS-C357974-20 20 µg (0.2 mg/ml) $354 
LS-C357974-100 100 µg (0.2 mg/ml) $550 
Actin Antibody - IHC testing of FFPE rhabdomyosarcoma with Muscle Actin antibody
Actin Antibody - Muscle Actin antibody HHF35 immunohistochemistry leiomyosarcoma 20X.  This image was taken for the unmodified form of this product. Other forms have not been tested.
Actin Antibody - Muscle Actin antibody HHF35 immunohistochemistry leiomyosarcoma 10X.  This image was taken for the unmodified form of this product. Other forms have not been tested.
Actin Antibody - Western blot testing of 1) human muscle and 2) mouse muscle cell lysate with Muscle Actin antibody (clone HHF35).
Actin Antibody - IHC testing of FFPE rhabdomyosarcoma with Muscle Actin antibody
Actin Antibody - Muscle Actin antibody HHF35 immunohistochemistry leiomyosarcoma 20X.  This image was taken for the unmodified form of this product. Other forms have not been tested.
Actin Antibody - Muscle Actin antibody HHF35 immunohistochemistry leiomyosarcoma 10X.  This image was taken for the unmodified form of this product. Other forms have not been tested.
Actin Antibody - Western blot testing of 1) human muscle and 2) mouse muscle cell lysate with Muscle Actin antibody (clone HHF35).
1 of 4
2 of 4
3 of 4
4 of 4

Monoclonal Mouse anti‑Human Actin Antibody (clone HHF35, IHC, IF, WB) LS‑C357974

Monoclonal Mouse anti‑Human Actin Antibody (clone HHF35, IHC, IF, WB) LS‑C357974

Antibody:
Actin Mouse anti-Human Monoclonal (HHF35) Antibody
Application:
IHC, IHC-P, IF, WB, IP, Flo
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$354
LS-C357974-20
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Actin Mouse anti-Human Monoclonal (HHF35) Antibody
Application:
IHC, IHC-P, IF, WB, IP, Flo
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
Actin antibody LS-C357974 is an unconjugated mouse monoclonal antibody to mouse Actin. Validated for Flow, IF, IHC, IP and WB.
Target
Human Actin
Host
Mouse
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
HHF35
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified. Also available Azide-free, BSA-free, Ready-to-Use.
Immunogen
SDS extract of human myocardium.
Specificity
Human Actin
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Immunofluorescence (0.5 - 1 µg/ml)
  • Western blot (0.5 - 1 µg/ml)
  • Immunoprecipitation
  • Flow Cytometry (0.5 - 1 µg/10E6 cells)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. This mAb recognizes alpha and gamma isotypes of all muscle groups. Nonmuscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of nonmuscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative. It stains tumors of smooth (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
Presentation
1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide
Storage
Store at 2°C to 8°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/25/2022