Human ACTA2 / Smooth Muscle Actin

Mouse

Human, Monkey, Mouse, Rat, Dog, Guinea pig, Rabbit, Chicken (tested or 100% immunogen sequence identity)

IgG2a,k Monoclonal

SPM332

Unconjugated

Protein G purified

Unmodified. Also available Azide-free, BSA-free.

10 AA peptide from the alpha smooth muscle isoform of actin.

Human ACTA2 / Smooth Muscle Actin

• IHC
• IHC - Paraffin (0.25 - 0.5 µg/ml)
• Immunofluorescence (0.5 - 1 µg/ml)
• Flow Cytometry (0.5 - 1 µg/10E6 cells)

Actin is a major component of the cytoskeleton and is present in most cell types. This mAb is highly specific to actin from smooth muscles. Its epitope lies in the first four N-terminal amino acids. This mAb does not stain cardiac or skeletal muscle. however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin. The optimal dilution of the Smooth Muscle Actin antibody for each application should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P62736 NCBI: NM_001613 NP_001604.1