Products
Research Areas
COVID-19
Resources
Contact Us
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Cart
How To Buy
Contact Us

Locations

Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464

Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers


Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us


Additional Contact Details

Distributors
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Antibodies > ACTA2 / Smooth Muscle Actin > LS-C800707
 
Print Page  
Catalog Number Size Price
LS-C800707-100 100 µl (1 mg/ml) $379 
LS-C800707-200 200 µl (1 mg/ml) $421 
ACTA2 / Smooth Muscle Actin Antibody - 1:100 staining rat lung tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ACTA2 / Smooth Muscle Actin Antibody - 1:100 staining human seminoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ACTA2 / Smooth Muscle Actin Antibody - 1:100 staining mouse kidney tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ACTA2 / Smooth Muscle Actin Antibody - 1:100 staining human appendix tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ACTA2 / Smooth Muscle Actin Antibody - 1:100 staining human skin tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ACTA2 / Smooth Muscle Actin Antibody - 1:100 staining mouse testis tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ACTA2 / Smooth Muscle Actin Antibody - The expression of a-SMA and a-Ac-Tub in lung tissue measured by IHC
ACTA2 / Smooth Muscle Actin Antibody - 1:100 staining rat uterine tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ACTA2 / Smooth Muscle Actin Antibody - 1:100 staining mouse pancreas tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ACTA2 / Smooth Muscle Actin Antibody - 1:100 staining rat gastric tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ACTA2 / Smooth Muscle Actin Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
ACTA2 / Smooth Muscle Actin Antibody - Western blot analysis on JK using alpha-SMA antibody. The lane on the left is blocked with the antigen-specific peptide.
ACTA2 / Smooth Muscle Actin Antibody - 1:100 staining rat lung tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ACTA2 / Smooth Muscle Actin Antibody - 1:100 staining human seminoma tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ACTA2 / Smooth Muscle Actin Antibody - 1:100 staining mouse kidney tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ACTA2 / Smooth Muscle Actin Antibody - 1:100 staining human appendix tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ACTA2 / Smooth Muscle Actin Antibody - 1:100 staining human skin tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ACTA2 / Smooth Muscle Actin Antibody - 1:100 staining mouse testis tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ACTA2 / Smooth Muscle Actin Antibody - The expression of a-SMA and a-Ac-Tub in lung tissue measured by IHC
ACTA2 / Smooth Muscle Actin Antibody - 1:100 staining rat uterine tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ACTA2 / Smooth Muscle Actin Antibody - 1:100 staining mouse pancreas tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ACTA2 / Smooth Muscle Actin Antibody - 1:100 staining rat gastric tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
ACTA2 / Smooth Muscle Actin Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
ACTA2 / Smooth Muscle Actin Antibody - Western blot analysis on JK using alpha-SMA antibody. The lane on the left is blocked with the antigen-specific peptide.
1 of 12
2 of 12
3 of 12
4 of 12
5 of 12
6 of 12
7 of 12
8 of 12
9 of 12
10 of 12
11 of 12
12 of 12

Polyclonal Rabbit anti‑Human ACTA2 / Smooth Muscle Actin Antibody (IHC, IF, WB) LS‑C800707

Polyclonal Rabbit anti‑Human ACTA2 / Smooth Muscle Actin Antibody (IHC, IF, WB) LS‑C800707

Submit Review LSBio Guarantee
Antibody:
ACTA2 / Smooth Muscle Actin Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, IP, Peptide-ELISA
Reactivity:
Human, Mouse, Rat, Bovine
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C800707-100
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
ACTA2 / Smooth Muscle Actin Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IF, WB, IP, Peptide-ELISA
Reactivity:
Human, Mouse, Rat, Bovine
Format:
Unconjugated, Unmodified

Specifications

Description
Smooth Muscle Actin antibody LS-C800707 is an unconjugated rabbit polyclonal antibody to Smooth Muscle Actin (ACTA2) from human. It is reactive with human, mouse, rat and other species. Validated for IF, IHC, IP, Peptide-ELISA and WB.
Target
Human ACTA2 / Smooth Muscle Actin
Synonyms
ACTA2 | ACTVS | Alpha-actin-2 | Alpha-cardiac actin | AAT6 | Actin, aortic smooth muscle | ACTSA | Smooth Muscle Actin | MYMY5 | SMA
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide affinity chromatography.
Modifications
Unmodified
Immunogen
Synthetic peptide corresponding to N-Terminus residues of Human alpha smooth muscle Actin.
Specificity
Alpha-SMA antibody detects endogenous levels of alpha-SMA.
Applications
  • IHC (1:50 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
  • Immunoprecipitation
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 150 mM NaCl, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ACTA2 / Smooth Muscle Actin
P62736 NM_001613 NP_001604.1

Publications (0)

Submit a Publication for LS-C800707

Customer Reviews (0)

Submit a review for LS-C800707

Featured Products

ACTA2 / Smooth Muscle Actin Antibody - Anti-ACTA2 antibody IHC of human vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
PathPlus™ ACTA2 / Smooth Muscle Actin Antibody (aa1‑50) LS‑B3933
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Pig, Rabbit, Chicken
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Product
ACTA2 / Smooth Muscle Actin Antibody (clone 1A4, PE) LS‑C124868
Species: Human, Mouse, Rat, Rabbit
Applications: Flow Cytometry
Product
ACTA2 / Smooth Muscle Actin Antibody (clone 1A4, APC) LS‑C182754
Species: Human
Applications: Flow Cytometry
ACTA2 / Smooth Muscle Actin Antibody - Anti-ACTA2 antibody IHC of human colon, vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC‑plus™ ACTA2 / Smooth Muscle Actin Antibody (N‑Terminus) LS‑B2506
Species: Human, Mouse, Rat, Bovine, Rabbit, Chicken
Applications: IHC, IHC - Paraffin
Product
ACTA2 / Smooth Muscle Actin Antibody (N‑Terminus, clone 1A4, Biotin) LS‑C86744
Species: Baboon
Applications: IHC, IHC - Paraffin, IHC - Frozen, Immunofluorescence
ACTA2 / Smooth Muscle Actin Antibody - Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)
IHC‑plus™ ACTA2 / Smooth Muscle Actin Antibody (clone 1H8) LS‑B12102
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry, ELISA
See all ACTA2 / Smooth Muscle Actin Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/27/2024
Family of Companies
Site Links Home All Antibodies How To Buy All Proteins Distributors All ELISA & Assay Kits Reviews PathPlus™ Antibodies
Follow LSBio View Cart Register or Login
Copyright © 2022 LifeSpan BioSciences, Inc - Privacy Policy
Contact Us

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464

Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

To place an order:
Orders@LSBio.com

For questions about orders:
Customer.Support@LSBio.com

For technical assistance:
Technical.Support@LSBio.com