CD143 antibody LS-C3716 is an unconjugated mouse monoclonal antibody to CD143 (ACE) from human, mouse, rat and other species. Validated for ELISA, IHC and WB.
Human, Monkey, Mouse, Rat, Bovine, Cat, Dog, Guinea pig, Rabbit
(tested or 100% immunogen sequence identity)
Tissue culture supernatant
- IHC - Paraffin
- Western blot (1:50 - 1:100)
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ACE / CD143 antibody was raised against denatured human kidney CD143 (Angiotensin converting enzyme).
Recognizes human CD143, also know as angiotensin-converting enzyme (ACE). CD143 exists in two forms, a 170kD somatic form and a 90kD germinal form. The somatic form is expressed by endothelial cells (especially those of lung capillaries and arterioles), epithelial cells (especially in proximal renal tubules and small intestine), by some neuronal cells and variably by some macrophages and T lymphocytes. The germinal form is expressed by spermatozoa. Study of CD143 may be relevant to fertility (especially sperm-egg interactions), atherosclerosis and in drug targeting to the lung. This clone recognizes denatured ACE, binding to an epitope in the C domain and has been reported to be active in Western blotting. Species cross-reactivity: Human, monkey, rabbit, bovine, canine, feline, guinea pig, rat, mouse.
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Western Blot: 1:50-1:100. Immunohistochemistry (Paraffin): Neat. 1:20. Requires antigen retrieval using heat treatment methods prior to staining. Positive control: Kidney (lung in rat studies).
Tissue Culture Supernatant. Supplied as a liquid, 0.1% sodium azide.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.