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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ACE / CD143 Antibody LS‑C3716
CD143 antibody LS-C3716 is an unconjugated mouse monoclonal antibody to CD143 (ACE) from human, mouse, rat and other species. Validated for ELISA, IHC and WB.
Catalog
Size
Price
LS-C3716-1000
1000 µl
$755
Description
CD143 antibody LS-C3716 is an unconjugated mouse monoclonal antibody to CD143 (ACE) from human, mouse, rat and other species. Validated for ELISA, IHC and WB.
Target
Human ACE / CD143
Host
Mouse
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Cat, Dog, Guinea pig, Rabbit (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Unconjugated
Purification
Tissue culture supernatant
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin
  • Western blot (1:50 - 1:100)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
ACE / CD143 antibody was raised against denatured human kidney CD143 (Angiotensin converting enzyme).
Specificity
Recognizes human CD143, also know as angiotensin-converting enzyme (ACE). CD143 exists in two forms, a 170kD somatic form and a 90kD germinal form. The somatic form is expressed by endothelial cells (especially those of lung capillaries and arterioles), epithelial cells (especially in proximal renal tubules and small intestine), by some neuronal cells and variably by some macrophages and T lymphocytes. The germinal form is expressed by spermatozoa. Study of CD143 may be relevant to fertility (especially sperm-egg interactions), atherosclerosis and in drug targeting to the lung. This clone recognizes denatured ACE, binding to an epitope in the C domain and has been reported to be active in Western blotting. Species cross-reactivity: Human, monkey, rabbit, bovine, canine, feline, guinea pig, rat, mouse.
Usage
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Western Blot: 1:50-1:100. Immunohistochemistry (Paraffin): Neat. 1:20. Requires antigen retrieval using heat treatment methods prior to staining. Positive control: Kidney (lung in rat studies).
Presentation
Tissue Culture Supernatant. Supplied as a liquid, 0.1% sodium azide.
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About ACE / CD143
P12821 NM_000789 NP_000780.1

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Requested From: United States
Date Requested: 12/14/2018
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